Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation

Baiyong Li; Hong Yu; Wei Ping Zheng; Reinhard Voll; Songqing Na; Andrew W. Roberts; David A. Williams; Roger J. Davis; Sankar Ghosh; Richard A. Flavell

doi:10.1126/science.288.5474.2219

Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation

Baiyong Li, Hong Yu, Wei Ping Zheng, Reinhard Voll, Songqing Na, Andrew W. Roberts, David A. Williams, Roger J. Davis, Sankar Ghosh, Richard A. Flavell

Research output: Contribution to journal › Article › peer-review

146 Scopus citations

Abstract

T helper 1 (T(H)1) cells mediate cellular immunity, whereas T(H)2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine T(H)1 cells. Rac induces the interferon-γ (IFN-γ) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-γ production. Dominant-negative Rac inhibited IFN-γ production in murine T cells. Moreover, T cells from Rac2^-/^- mice showed decreased IFN-γ production under T(H)1 conditions in vitro. Thus, Rac2 activates T(H)1-specific signaling and IFN-γ gene expression.

Original language	English (US)
Pages (from-to)	2219-2222
Number of pages	4
Journal	Science
Volume	288
Issue number	5474
DOIs	https://doi.org/10.1126/science.288.5474.2219
State	Published - Jun 23 2000
Externally published	Yes

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@article{2f32e415c59b4c4a8d57c8152f6a0982,

title = "Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation",

abstract = "T helper 1 (T(H)1) cells mediate cellular immunity, whereas T(H)2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine T(H)1 cells. Rac induces the interferon-γ (IFN-γ) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-γ production. Dominant-negative Rac inhibited IFN-γ production in murine T cells. Moreover, T cells from Rac2-/- mice showed decreased IFN-γ production under T(H)1 conditions in vitro. Thus, Rac2 activates T(H)1-specific signaling and IFN-γ gene expression.",

author = "Baiyong Li and Hong Yu and Zheng, \{Wei Ping\} and Reinhard Voll and Songqing Na and Roberts, \{Andrew W.\} and Williams, \{David A.\} and Davis, \{Roger J.\} and Sankar Ghosh and Flavell, \{Richard A.\}",

year = "2000",

month = jun,

day = "23",

doi = "10.1126/science.288.5474.2219",

language = "English (US)",

volume = "288",

pages = "2219--2222",

journal = "Science",

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publisher = "American Association for the Advancement of Science",

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TY - JOUR

T1 - Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation

AU - Li, Baiyong

AU - Yu, Hong

AU - Zheng, Wei Ping

AU - Voll, Reinhard

AU - Na, Songqing

AU - Roberts, Andrew W.

AU - Williams, David A.

AU - Davis, Roger J.

AU - Ghosh, Sankar

AU - Flavell, Richard A.

PY - 2000/6/23

Y1 - 2000/6/23

N2 - T helper 1 (T(H)1) cells mediate cellular immunity, whereas T(H)2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine T(H)1 cells. Rac induces the interferon-γ (IFN-γ) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-γ production. Dominant-negative Rac inhibited IFN-γ production in murine T cells. Moreover, T cells from Rac2-/- mice showed decreased IFN-γ production under T(H)1 conditions in vitro. Thus, Rac2 activates T(H)1-specific signaling and IFN-γ gene expression.

AB - T helper 1 (T(H)1) cells mediate cellular immunity, whereas T(H)2 cells potentiate antiparasite and humoral immunity. We used a complementary DNA subtraction method, representational display analysis, to show that the small guanosine triphosphatase Rac2 is expressed selectively in murine T(H)1 cells. Rac induces the interferon-γ (IFN-γ) promoter through cooperative activation of the nuclear factor kappa B and p38 mitogen-activated protein kinase pathways. Tetracycline-regulated transgenic mice expressing constitutively active Rac2 in T cells exhibited enhanced IFN-γ production. Dominant-negative Rac inhibited IFN-γ production in murine T cells. Moreover, T cells from Rac2-/- mice showed decreased IFN-γ production under T(H)1 conditions in vitro. Thus, Rac2 activates T(H)1-specific signaling and IFN-γ gene expression.

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U2 - 10.1126/science.288.5474.2219

DO - 10.1126/science.288.5474.2219

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AN - SCOPUS:0034705628

SN - 0036-8075

VL - 288

SP - 2219

EP - 2222

JO - Science

JF - Science

IS - 5474

ER -

Role of the guanosine triphosphatase Rac2 in T helper 1 cell differentiation

Abstract

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