Role of pinoline and melatonin in stabilizing hepatic microsomal membranes against oxidative stress

J. J. García, R. J. Reiter, J. Pié, G. G. Ortiz, J. Cabrera, R. M. Sáinz, D. Acuña-Castroviejo

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

We investigated the influence of pinoline (0.01-1.5 mM) on microsomal membrane fluidity before and after rigidity was induced by oxidative stress. In addition, we tested the effect of pinoline in the presence of 1 mM melatonin. The fluidity in rat hepatic microsomes was monitored using fluorescence spectroscopy and it was compared to the inhibition of malonaldehyde (MDA) plus 4-hydroxyalkenals (4-HDA) production as a reflection of lipid peroxidation. Below 0.6 mM, pinoline inhibited membrane rigidity in a manner parallel to its inhibitory effect on MDA + 4-HDA formation. At concentrations between 1-1.5 mM, pinoline was less effective in stabilizing microsomal membranes than was predicted from its inhibition of lipid peroxidation. The addition of 1 mM melatonin enhanced the membrane- stabilizing activity of pinoline (0.01-0.6 mM). This cooperative effect was not observed for concentrations of pinoline between 1-1.5 mM. When pinoline was tested without induced oxidative damage, 1-1.5 mM pinoline maintained membrane fluidity at the same level as that recorded after induced lipid peroxidation. The results suggest that pinoline may be another pineal molecule that prevents membrane rigidity mediated by lipid peroxidation and this ability is enhanced by melatonin.

Original languageEnglish (US)
Pages (from-to)609-616
Number of pages8
JournalJournal of Bioenergetics and Biomembranes
Volume31
Issue number6
DOIs
StatePublished - Dec 1 1999

Keywords

  • Lipid peroxidation
  • Melatonin
  • Membrane fluidity
  • Microsome
  • Pinoline

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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