RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex

M. Kronenberg, M. Steinmetz, J. Kobori, Ellen B Kraig, J. A. Kapp, C. W. Pierce, C. M. Sorensen, G. Suzuki, T. Tada, L. Hood

Research output: Contribution to journalArticle

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Abstract

The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.

Original languageEnglish (US)
Pages (from-to)5704-5708
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume80
Issue number181
StatePublished - 1983
Externally publishedYes

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Major Histocompatibility Complex
T-Lymphocytes
Peptides
Cosmids
Gene Rearrangement
Hybridomas
Spermatozoa
Epitopes
Clone Cells
DNA
Genes
immune RNA

ASJC Scopus subject areas

  • Genetics
  • General

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RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex. / Kronenberg, M.; Steinmetz, M.; Kobori, J.; Kraig, Ellen B; Kapp, J. A.; Pierce, C. W.; Sorensen, C. M.; Suzuki, G.; Tada, T.; Hood, L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 80, No. 181, 1983, p. 5704-5708.

Research output: Contribution to journalArticle

Kronenberg, M. ; Steinmetz, M. ; Kobori, J. ; Kraig, Ellen B ; Kapp, J. A. ; Pierce, C. W. ; Sorensen, C. M. ; Suzuki, G. ; Tada, T. ; Hood, L. / RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex. In: Proceedings of the National Academy of Sciences of the United States of America. 1983 ; Vol. 80, No. 181. pp. 5704-5708.
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abstract = "The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.",
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T1 - RNA transcripts for I-J polypeptides are apparently not encoded between the I-A and I-E subregions of the murine major histocompatibility complex

AU - Kronenberg, M.

AU - Steinmetz, M.

AU - Kobori, J.

AU - Kraig, Ellen B

AU - Kapp, J. A.

AU - Pierce, C. W.

AU - Sorensen, C. M.

AU - Suzuki, G.

AU - Tada, T.

AU - Hood, L.

PY - 1983

Y1 - 1983

N2 - The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.

AB - The I-J subregion of the mouse major histocompatibility complex has been reported to encode antigenic determinants expressed by suppressor T cells. Previously, cosmid clones were obtained from mouse sperm DNA that contain all of the sequences between the I-A and I-E subregions, where I-J has been mapped genetically. However, hybridization of these sequences to RNA prepared from several I-J-positive suppressor T-cell hybridomas did not reveal the presence of a transcript. In addition, no rearrangements in this DNA were detected in the suppressor T cells that we have analyzed. Our results indicate that the I-J polypeptides are not encoded between the I-A and I-E subregions of the major histocompatibility complex. We discuss several hypotheses concerning the possible location and expression of I-J genes.

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