RNA polymerase activity is associated with viral particles isolated from Leishmania braziliensis subsp. guyanensis

G. Widmer, M. C. Keenan, J. L. Patterson

    Research output: Contribution to journalArticlepeer-review

    28 Scopus citations

    Abstract

    Viral particles purified from species of the protozoan parasite Leishmania braziliensis subsp. guyanensis by centrifugation in CsCl gradients were examined for the presence of viral polymerase. We demonstrated that RNA-dependent RNA polymerase is associated with viral particles. Viral transcription was studied in vitro with pulse-chase experiments and by assaying the RNase sensitivity of the viral transcripts. Viral polymerase synthesized full-length transcripts within 1 h. Double-stranded, genome-length, and single-stranded RNAs were produced in this system. The nature of the RNA extracted from virions was also tested by RNase protection assays; both single-stranded and double-stranded RNAs were found.

    Original languageEnglish (US)
    Pages (from-to)3712-3715
    Number of pages4
    JournalJournal of virology
    Volume64
    Issue number8
    DOIs
    StatePublished - 1990

    ASJC Scopus subject areas

    • Microbiology
    • Immunology
    • Insect Science
    • Virology

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