RNA polymerase activity in isolated nuclei from eggs of Oncopeltus fasciatus

S. E. Harris; H. S. Forrest

doi:10.1016/0022-1910(71)90214-9

RNA polymerase activity in isolated nuclei from eggs of Oncopeltus fasciatus

S. E. Harris, H. S. Forrest

Department of Periodontics

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Nuclei, isolated from various developmental stages of the eggs of the milkweed bug Oncopeltus fasciatus, have been assayed for RNA polymerase activity in the presence of Mg²⁺ ions and in a low salt environment. This activity changes during development. These changes can be correlated with in vivo changes in the rate of RNA synthesis. The base composition of the RNA transcribed in vitro is intermediate between ribosomal RNA and DNA-like RNA.

Original language	English (US)
Pages (from-to)	303-312
Number of pages	10
Journal	Journal of Insect Physiology
Volume	17
Issue number	2
DOIs	https://doi.org/10.1016/0022-1910(71)90214-9
State	Published - Feb 1971

ASJC Scopus subject areas

Physiology
Insect Science

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10.1016/0022-1910(71)90214-9

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title = "RNA polymerase activity in isolated nuclei from eggs of Oncopeltus fasciatus",

abstract = "Nuclei, isolated from various developmental stages of the eggs of the milkweed bug Oncopeltus fasciatus, have been assayed for RNA polymerase activity in the presence of Mg2+ ions and in a low salt environment. This activity changes during development. These changes can be correlated with in vivo changes in the rate of RNA synthesis. The base composition of the RNA transcribed in vitro is intermediate between ribosomal RNA and DNA-like RNA.",

author = "Harris, {S. E.} and Forrest, {H. S.}",

note = "Funding Information: work was supported by grants from the U.S. Public Health GM00337) and by the Robert A. Welch Foundation, Houston,",

year = "1971",

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doi = "10.1016/0022-1910(71)90214-9",

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T1 - RNA polymerase activity in isolated nuclei from eggs of Oncopeltus fasciatus

AU - Harris, S. E.

AU - Forrest, H. S.

N1 - Funding Information: work was supported by grants from the U.S. Public Health GM00337) and by the Robert A. Welch Foundation, Houston,

PY - 1971/2

Y1 - 1971/2

N2 - Nuclei, isolated from various developmental stages of the eggs of the milkweed bug Oncopeltus fasciatus, have been assayed for RNA polymerase activity in the presence of Mg2+ ions and in a low salt environment. This activity changes during development. These changes can be correlated with in vivo changes in the rate of RNA synthesis. The base composition of the RNA transcribed in vitro is intermediate between ribosomal RNA and DNA-like RNA.

AB - Nuclei, isolated from various developmental stages of the eggs of the milkweed bug Oncopeltus fasciatus, have been assayed for RNA polymerase activity in the presence of Mg2+ ions and in a low salt environment. This activity changes during development. These changes can be correlated with in vivo changes in the rate of RNA synthesis. The base composition of the RNA transcribed in vitro is intermediate between ribosomal RNA and DNA-like RNA.

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ER -

RNA polymerase activity in isolated nuclei from eggs of Oncopeltus fasciatus

Abstract

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