Rhodanese can partially refold in its GroEL-GroES-ADP complex and can be released to give a homogeneous product

Anusri Mitra Bhattacharyya, Paul M. Horowitz

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Molecular chaperones GroEL and GroES facilitate reactivation of denatured rhodanese which folds poorly unless the process is assisted. The present work tests the hypothesis that more extensively unfolded forms of rhodanese bind tighter than those forms that appear later in the folding pathway. The study of the interaction of different urea-induced forms of rhodanese with GroEL suggests that species preceding the domain folded form bind directly and productively to GroEL. Rhodanese partially folds while in the GroEL-GroES-ADP complex, but it does not significantly reach an active state. Partially folded rhodanese can be released from the GroEL-GroES-ADP complex by subdenaturing concentrations of urea as a homogeneous species that is committed to fold to the native conformation with little or no partitioning to the aggregated state. Dilution of denatured rhodanese to the same final concentration gives less active enzyme and significant aggregation. Urea denaturation studies show that active rhodanese released from complexes behaves identically to native enzyme, while spontaneously folded rhodanese has a different stability. These results are interpreted using a previously proposed model based on studies of unassisted rhodanese folding [Gorovits, B. M., McGee, W. A., and Horowitz, P. M. (1998) Biochim. Biophys. Acta 1382, 120-128. Panda, M., Gorovits, B. M., and Horowitz, P. M. (2000) J. Biol. Chem. 275, 63-70].

Original languageEnglish (US)
Pages (from-to)2421-2428
Number of pages8
Issue number7
StatePublished - Feb 19 2002
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry


Dive into the research topics of 'Rhodanese can partially refold in its GroEL-GroES-ADP complex and can be released to give a homogeneous product'. Together they form a unique fingerprint.

Cite this