RhoB-dependent modulation of postendocytic traffic in polarized madin-darby canine kidney cells

Christine Rondanino, Raul Rojas, Wily G. Ruiz, Exing Wang, Rebecca P. Hughey, Kenneth W. Dunn, Gerard Apodaca

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


The Rho family of GTPases is implicated in the control of endocytic and biosynthetic traffic of many cell types; however, the cellular distribution of RhoB remains controversial and its function is not well understood. Using confocal microscopy, we found that endogenous RhoB and green fluorescent protein-tagged wild-type RhoB were localized to early endosomes, and to a much lesser extent to recycling endosomes, late endosomes or Golgi complex of fixed or live polarized Madin-Darby canine kidney cells. Consistent with RhoB localization to early endosomes, we observed that expression of dominant-negative RhoBN19 or dominant-active RhoBV14 altered postendocytic traffic of ligand-receptor complexes that undergo recycling, degradation or transcytosis. In vitro assays established that RhoB modulated the basolateral-to-apical transcytotic pathway by regulating cargo exit from basolateral early endosomes. Our results indicate that RhoB is localized, in part, to early endosomes where it regulates receptor egress through the early endocytic system.

Original languageEnglish (US)
Pages (from-to)932-949
Number of pages18
Issue number7
StatePublished - Jul 2007
Externally publishedYes


  • Early endosomes
  • Endocytic traffic
  • Endocytosis
  • MDCK
  • RhoB
  • Transcytosis

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Structural Biology
  • Biochemistry
  • Cell Biology


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