Retroviral suicide vector does not inhibit neointimal growth in a porcine coronary model of restenosis

R. Wayne Barbee, Diane E. Madras, Richard N. Ré, Joseph P. Murgo, William D. Davenport, Jason Giardina, Julia L. Cook

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

We attempted to attenuate neointimal formation following vascular injury using a retroviral suicide vector. Epicardial coronary arteries of adult miniature swine were injured by deployment of oversized tantalum stents. One week later, injured segments were exposed to packaged retroviral constructs with or without the herpes simplex virus thymidine kinase gene. Ganciclovir treatments were initiated 48 hours later in both control and experimental swine and continued for two weeks. Four weeks following vascular injury, both control and experimental arteries were harvested and histologically prepared for image analysis. Despite adequate marker gene expression, there was no significant difference in the neointimal area or neointimal/media ratio between control and experimental groups. While the HSVtk ganciclovir system attenuates cell proliferation in other systems, retroviral vector targeting of vascular smooth muscle cells for elimination may be too inefficient to prevent restenosis following angioplasty.

Original languageEnglish (US)
Pages (from-to)89-98
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume207
Issue number1
DOIs
StatePublished - Feb 6 1995
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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