Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1

Yoshihiro Sowa, Yuzuru Shiio, Tsuyoshi Fujita, Takuji Matsumoto, Yusuke Okuyama, Daishiro Kato, Jun Ichiro Inoue, Jun Ichi Sawada, Masahide Goto, Hajime Watanabe, Hiroshi Handa, Toshiyuki Sakai

Research output: Contribution to journalArticle

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Abstract

We previously reported two oncogenic point mutations present in the RB (retinoblastoma) gene promoter region, found at consensus Spl and ATF sites, respectively, and in two separate hereditary RB families. However, Spl protein was shown not to bind to the Spl site; this indicated that the Spl consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TFI on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1.

Original languageEnglish (US)
Pages (from-to)3145-3148
Number of pages4
JournalCancer Research
Volume57
Issue number15
StatePublished - 1997
Externally publishedYes

Fingerprint

Retinoblastoma Genes
Retinoblastoma
Genetic Promoter Regions
Transcription Factors
Trans-Activators
Point Mutation
Adenoviridae
Transcriptional Activation
Mutation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Sowa, Y., Shiio, Y., Fujita, T., Matsumoto, T., Okuyama, Y., Kato, D., ... Sakai, T. (1997). Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1. Cancer Research, 57(15), 3145-3148.

Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1. / Sowa, Yoshihiro; Shiio, Yuzuru; Fujita, Tsuyoshi; Matsumoto, Takuji; Okuyama, Yusuke; Kato, Daishiro; Inoue, Jun Ichiro; Sawada, Jun Ichi; Goto, Masahide; Watanabe, Hajime; Handa, Hiroshi; Sakai, Toshiyuki.

In: Cancer Research, Vol. 57, No. 15, 1997, p. 3145-3148.

Research output: Contribution to journalArticle

Sowa, Y, Shiio, Y, Fujita, T, Matsumoto, T, Okuyama, Y, Kato, D, Inoue, JI, Sawada, JI, Goto, M, Watanabe, H, Handa, H & Sakai, T 1997, 'Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1', Cancer Research, vol. 57, no. 15, pp. 3145-3148.
Sowa, Yoshihiro ; Shiio, Yuzuru ; Fujita, Tsuyoshi ; Matsumoto, Takuji ; Okuyama, Yusuke ; Kato, Daishiro ; Inoue, Jun Ichiro ; Sawada, Jun Ichi ; Goto, Masahide ; Watanabe, Hajime ; Handa, Hiroshi ; Sakai, Toshiyuki. / Retinoblastoma binding factor 1 site in the core promoter region of the human RB gene is activated by hGABP/E4TF1. In: Cancer Research. 1997 ; Vol. 57, No. 15. pp. 3145-3148.
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AU - Sakai, Toshiyuki

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AB - We previously reported two oncogenic point mutations present in the RB (retinoblastoma) gene promoter region, found at consensus Spl and ATF sites, respectively, and in two separate hereditary RB families. However, Spl protein was shown not to bind to the Spl site; this indicated that the Spl consensus site mutation was blocking the action of an alternative transcription factor, which we called RBF-1 (retinoblastoma binding factor 1). Subsequent purification of RBF-1 revealed it to be hGABP/E4TF1, a transactivator from the adenovirus early-region 4 promoter. In this study, we directly examined the effects of hGABP/E4TFI on transactivation of the RB gene promoter through the RBF-1 site. As expected, hGABP/E4TF1 enhanced the core RB promoter activity, whereas it did not stimulate a mutant RBF-1 site. We therefore conclude that the most essential transcription factor in the human RB gene is likely to be hGABP/E4TF1.

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