Retinal function assessed by ERG before and after induction of ocular aspergillosis and treatment by the anti-fungal, micafungin, in rabbits

Joseph M. Harrison, Randolph D. Glickman, Charles S. Ballentine, Yolanda Trigo, Melanie A. Pena, Pearl Kurian, Laura K. Najvar, Neeru Kumar, Ankit H. Patel, William E. Sponsel, John R. Graybill, William C. Lloyd, Margaret M. Miller, Gianmarco Paris, Fernando Trujillo, Aaron Miller, Robert Melendez

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

This study was conducted to evaluate the effectiveness of a new antifungal drug, micafungin, and standard antifungal drugs against endophthalmitis induced in a rabbit by intravitreal injection of Aspergillus fumigatus, an important fungal pathogen. Effectiveness was evaluated by the preservation of b-wave amplitude at 72 h after injection of the fungus relative to the b-wave amplitude at baseline before any intravitreal injections. A 0.06 ml inoculum of 106 conidia of A. fumigatus was injected into the vitreous of the right eye of all rabbits; and, 12 h later, a 0.06 ml solution containing one of 3 antifungal drugs or saline was injected into the vitreous of both eyes. All three antifungal drugs produced significant b-wave preservation at 72 h in infected eyes compared to that in infected eyes receiving saline injections. There was no statistically significant difference between the effects of micafungin and amphotericin B in the right eyes with fungal endophthalmitis, and both produced significantly more preservation of b-wave amplitude than voriconazole. Amphotericin B, but neither micafungin nor voriconazole produced significant reduction of the b-wave amplitude in the left eyes.

Original languageEnglish (US)
Pages (from-to)37-55
Number of pages19
JournalDocumenta Ophthalmologica
Volume110
Issue number1
DOIs
StatePublished - Jan 2005

Keywords

  • Amphotericin B
  • Aspergillus fumigatus
  • Electroretinogram
  • Endophthalmitis micafungin
  • Voriconazole

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Physiology (medical)

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    Harrison, J. M., Glickman, R. D., Ballentine, C. S., Trigo, Y., Pena, M. A., Kurian, P., Najvar, L. K., Kumar, N., Patel, A. H., Sponsel, W. E., Graybill, J. R., Lloyd, W. C., Miller, M. M., Paris, G., Trujillo, F., Miller, A., & Melendez, R. (2005). Retinal function assessed by ERG before and after induction of ocular aspergillosis and treatment by the anti-fungal, micafungin, in rabbits. Documenta Ophthalmologica, 110(1), 37-55. https://doi.org/10.1007/s10633-005-7342-0