Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E

Hong Yang, L. Jackson Roberts, Jian Shi Ming, Chun Zhou Li, Billy R. Ballard, Arlan Richardson, Mao Guo Zhong

Research output: Contribution to journalArticle

157 Citations (Scopus)

Abstract

Oxidative stress has been suggested to potentiate atherogenesis. However, studies that have investigated the effect of antioxidants on atherosclerosis showed inconsistent results, ie, atherosclerosis was either retarded or not changed by dietary antioxidants. This report directly examined the effect of overexpressing Cu/Zn-superoxide dismutase (Cu/Zn-SOD) and/or catalase on atherosclerosis and lipid peroxidation in mice lacking apolipoprotein E (ApoE-/-). Based on lipid staining of the en face of the aorta tree and the serial sections of the proximal aorta, ApoE-/- mice overexpressing catalase or both Cu/Zn-SOD and catalase had smaller and relatively early stages of atherosclerotic lesions (eg, foam cells and free lipids) when compared with ApoE-/- mice, who developed more advanced lesions (eg, fibrous caps and acellular areas). In addition, the retarded development of atherosclerosis was correlated with a reduced F 2-isoprostanes in the plasma and aortas in ApoE-/- mice overexpressing catalase or both Cu/Zn-SOD and catalase. In contrast, the levels of F2-isoprostanes and atherosclerosis in the ApoE-/- mice overexpressing Cu/Zn-SOD alone were comparable to ApoE-/- control mice. These observations implied that endogenously produced hydrogen peroxide, but not superoxide anions, contributed to the formation of oxidized lipids and the development of atherosclerosis in ApoE-/- mice.

Original languageEnglish (US)
Pages (from-to)1075-1081
Number of pages7
JournalCirculation Research
Volume95
Issue number11
DOIs
StatePublished - Nov 26 2004

Fingerprint

Apolipoproteins E
Catalase
Atherosclerosis
Aorta
Lipids
Antioxidants
F2-Isoprostanes
Isoprostanes
Foam Cells
Superoxide Dismutase-1
Superoxides
Hydrogen Peroxide
Lipid Peroxidation
Oxidative Stress
Staining and Labeling

Keywords

  • Atherosclerosis
  • Catalase
  • Cu/Zn-superoxide dismutase
  • F- isoprostanes

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E. / Yang, Hong; Roberts, L. Jackson; Ming, Jian Shi; Li, Chun Zhou; Ballard, Billy R.; Richardson, Arlan; Zhong, Mao Guo.

In: Circulation Research, Vol. 95, No. 11, 26.11.2004, p. 1075-1081.

Research output: Contribution to journalArticle

Yang, Hong ; Roberts, L. Jackson ; Ming, Jian Shi ; Li, Chun Zhou ; Ballard, Billy R. ; Richardson, Arlan ; Zhong, Mao Guo. / Retardation of atherosclerosis by overexpression of catalase or both Cu/Zn-superoxide dismutase and catalase in mice lacking apolipoprotein E. In: Circulation Research. 2004 ; Vol. 95, No. 11. pp. 1075-1081.
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