TY - JOUR
T1 - Restricted T cell receptor repertoire for acetylcholine receptor in murine myasthenia gravis
AU - Kraig, Ellen
AU - Pierce, Jessica L.
AU - Clarkin, Kimberly Z.
AU - Standifer, Nathan E.
AU - Currier, Patricia
AU - Wall, Katherine A.
AU - Infante, Anthony J.
N1 - Funding Information:
The authors wish to thank Dr. Keith Krolick and his laboratory for kindly providing the purified Torpedo acetylcholine receptor, Ms. Ji-yang Hu for synthesizing and purifying the AChR peptides used, Ms. Peggy Rifleman for amino acid analysis, and Dr. Sue Weintraub for mass spectroscopic analysis. This work was supported by Grants NS29093 (A.J.I.) and AI33195 (A.J.I., E.K.) from the NIH.
PY - 1996/12
Y1 - 1996/12
N2 - Immunization of C57BL/6 mice with AChR provokes symptoms similar to those seen in the disease myasthenia gravis. To elucidate the structural requirements for T cell recognition of AChR and to identify TcR features which might provide targets for immunotherapy, a panel of T cell hybridomas was generated after immunization of mice with the immunodominant peptide of the AChR α chain. The TcR genes expressed by these hybridomas were sequenced. TcR-Vβ6 was preferentially employed, but other Vβ genes were also observed. A conserved acidic residue was present in all CDR3 regions, regardless of the Vβ. The TcR-Vα repertoire was somewhat skewed with three Vα families accounting for 82% of the sequences. The utilization of multiple T cell receptor Vβ genes may contribute to the inability to inhibit EAMG by elimination of Vβ6+ T cells.
AB - Immunization of C57BL/6 mice with AChR provokes symptoms similar to those seen in the disease myasthenia gravis. To elucidate the structural requirements for T cell recognition of AChR and to identify TcR features which might provide targets for immunotherapy, a panel of T cell hybridomas was generated after immunization of mice with the immunodominant peptide of the AChR α chain. The TcR genes expressed by these hybridomas were sequenced. TcR-Vβ6 was preferentially employed, but other Vβ genes were also observed. A conserved acidic residue was present in all CDR3 regions, regardless of the Vβ. The TcR-Vα repertoire was somewhat skewed with three Vα families accounting for 82% of the sequences. The utilization of multiple T cell receptor Vβ genes may contribute to the inability to inhibit EAMG by elimination of Vβ6+ T cells.
KW - Myasthenia gravis
KW - T cell antigen receptor
KW - acetylcholine receptor
KW - immune repertoire
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U2 - 10.1016/S0165-5728(96)00151-8
DO - 10.1016/S0165-5728(96)00151-8
M3 - Article
C2 - 8982107
AN - SCOPUS:0030560756
SN - 0165-5728
VL - 71
SP - 87
EP - 95
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -