Restricted T cell receptor repertoire for acetylcholine receptor in murine myasthenia gravis

Ellen Kraig, Jessica L. Pierce, Kimberly Z. Clarkin, Nathan E. Standifer, Patricia Currier, Katherine A. Wall, Anthony J. Infante

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Immunization of C57BL/6 mice with AChR provokes symptoms similar to those seen in the disease myasthenia gravis. To elucidate the structural requirements for T cell recognition of AChR and to identify TcR features which might provide targets for immunotherapy, a panel of T cell hybridomas was generated after immunization of mice with the immunodominant peptide of the AChR α chain. The TcR genes expressed by these hybridomas were sequenced. TcR-Vβ6 was preferentially employed, but other Vβ genes were also observed. A conserved acidic residue was present in all CDR3 regions, regardless of the Vβ. The TcR-Vα repertoire was somewhat skewed with three Vα families accounting for 82% of the sequences. The utilization of multiple T cell receptor Vβ genes may contribute to the inability to inhibit EAMG by elimination of Vβ6+ T cells.

Original languageEnglish (US)
Pages (from-to)87-95
Number of pages9
JournalJournal of Neuroimmunology
Volume71
Issue number1-2
DOIs
StatePublished - Dec 1 1996

Keywords

  • Myasthenia gravis
  • T cell antigen receptor
  • acetylcholine receptor
  • immune repertoire

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Neurology
  • Clinical Neurology

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