Abstract
Optimal conditions for studying phytohemagglutinin (PHA)-induced transformation of baboon peripheral blood lymphocytes were determined. Maximal stimulation, as determined by uptake and incorporation of tritiated thymidine (total cell and trichloroacetic-acid-precipitable radioactivity), occurred at a PHA level of 12.5 μg in a culture volume of 0.25 ml containing 2 X 105 lymphocytes. Optimal stimulation occurred after a total incubation of 114 h, the last 18 h of which was in the presence of 1 μCi of the labeled DNA precursor per culture. While there was considerable variation in the extent of responsiveness of lymphocytes from individual animals, the shape of the dose-response and time-course curves for most mitogen concentrations was generally similar.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 298-308 |
| Number of pages | 11 |
| Journal | Journal of medical primatology |
| Volume | 6 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1977 |
| Externally published | Yes |
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)