Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophage in vitro

D. P. Krieg, R. J. Helmke, V. F. German, J. A. Mangos

Research output: Contribution to journalArticle

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Abstract

A unique, recently described rat alveolar macrophage cell line (NR8383) was used to study the interaction of the pulmonary immune system with a mucoid cystic fibrosis isolated of Pseudomonas aeruginosa (SRM-3), its nonmucoid revertant (SRM-3R), and a non-cystic fibrosis isolate (PAO-1). Strain SRM-3 was cultivated in a chemostat system to allow maintenance of an entirely mucoid population. The alveolar macrophage response to the mucoid and nonmucoid strains of P. aeuruginosa was determined by visually quantitating phagocytosis in acridine orange-stained monolayers and measuring the induction of an oxidative burst as indicated by chemiluminescence and H2O2 production. In all experiments, fewer than 2% of the NR8383 cells engulfed the mucoid SRM-3 isolate, while SRM-3R and PAO-1 were phagocytized by 15 and 41%, respectively. Opsonization by normal serum (complement) provided minimal phagocytic enhancement of these strains, whereas specific anti-P. aeruginosa antibody slightly elevated phagocytic responses to strains with nonmucoid phenotypes while providing a sevenfold increase in uptake of SRM-3. Chemiluminescent and H2O2 responses were comparable with the levels of phagocytosis observed, with very little or no response to the mucoid strain SRM-3. The data indicate that the strains with mucoid phenotypes are refractile to ingestion and that studies which describe ingestion of mucoid strains were likely measuring ingestion of revertants. Alginic acid (2 mg/ml) was found to inhibit stimulation of macrophages response to the opsonized and unopsonized nonmucoid strain PAO-1.

Original languageEnglish (US)
Pages (from-to)3173-3179
Number of pages7
JournalInfection and Immunity
Volume56
Issue number12
StatePublished - 1988
Externally publishedYes

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Alveolar Macrophages
Phagocytosis
Pseudomonas aeruginosa
Eating
Alveolar Epithelial Cells
Phenotype
Acridine Orange
Respiratory Burst
Luminescence
Cystic Fibrosis
Immune System
Fibrosis
Macrophages
Maintenance
Cell Line
Lung
Antibodies
Serum
Population
In Vitro Techniques

ASJC Scopus subject areas

  • Immunology

Cite this

Krieg, D. P., Helmke, R. J., German, V. F., & Mangos, J. A. (1988). Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophage in vitro. Infection and Immunity, 56(12), 3173-3179.

Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophage in vitro. / Krieg, D. P.; Helmke, R. J.; German, V. F.; Mangos, J. A.

In: Infection and Immunity, Vol. 56, No. 12, 1988, p. 3173-3179.

Research output: Contribution to journalArticle

Krieg, DP, Helmke, RJ, German, VF & Mangos, JA 1988, 'Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophage in vitro', Infection and Immunity, vol. 56, no. 12, pp. 3173-3179.
Krieg, D. P. ; Helmke, R. J. ; German, V. F. ; Mangos, J. A. / Resistance of mucoid Pseudomonas aeruginosa to nonopsonic phagocytosis by alveolar macrophage in vitro. In: Infection and Immunity. 1988 ; Vol. 56, No. 12. pp. 3173-3179.
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