Rescue of a positive stranded RNA virus from antigen negative neuroblastoma cells

S. A. Stohlman, Alan Y Sakaguchi, L. P. Weiner

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Single cell clones of latently infected mouse neuroblastoma cells were isolated from a culture chronically infected with mouse hepatitis virus in the presence of an antiviral antibody. These cell clones did not produce infections virus or exhibit viral cytopathic effects during cultivation at 32, 37, or 39°C. Infectious virus was isolated from single cell clones via fusion with permissive cells using polyethylene glycol, but not after fusion with inactivated Sendai virus or following treatment with metabolic inhibitors. One cell clone (S-3) from which virus was rescued was negative for viral antigen by immunofluorescence. The S-3 cell clone and no demonstrable virus antigen by complement-fixation tests using cytoplasmic extracts or virus-specified proteins detectable by polyacrylamide gel electrophoresis. The rescued viruses exhibited a temperature dependent growth defect at 32°C and have been classified as cold sensitive mutants. This study suggests that a complete genome of a positive stranded RNA virus can remain latent in infected cells without the expression of detectable virus antigen.

Original languageEnglish (US)
Pages (from-to)1029-1035
Number of pages7
JournalLife Sciences
Volume24
Issue number11
DOIs
StatePublished - Mar 12 1979
Externally publishedYes

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RNA Viruses
Neuroblastoma
Viruses
Clone cells
RNA
Antigens
Clone Cells
Viral Cytopathogenic Effect
Murine hepatitis virus
Sendai virus
Complement Fixation Tests
Viral Antigens
Virus Diseases
Fusion reactions
Antiviral Agents
Fluorescent Antibody Technique
Polyacrylamide Gel Electrophoresis
Genome
Electrophoresis
Temperature

ASJC Scopus subject areas

  • Pharmacology

Cite this

Rescue of a positive stranded RNA virus from antigen negative neuroblastoma cells. / Stohlman, S. A.; Sakaguchi, Alan Y; Weiner, L. P.

In: Life Sciences, Vol. 24, No. 11, 12.03.1979, p. 1029-1035.

Research output: Contribution to journalArticle

Stohlman, S. A. ; Sakaguchi, Alan Y ; Weiner, L. P. / Rescue of a positive stranded RNA virus from antigen negative neuroblastoma cells. In: Life Sciences. 1979 ; Vol. 24, No. 11. pp. 1029-1035.
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