Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calcium-sensing receptor

Osvaldo Rey, Steven H. Young, Romeo Papazyan, Mark S Shapiro, Enrique Rozengurt

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The calcium-sensing receptor (CaR) is an allosteric protein that responds to extracellular Ca2+ ([Ca2+]o) and aromatic amino acids with the production of different patterns of oscillations in intracellular Ca2+ concentration ([Ca2+]i). An increase in [Ca2+]o stimulates phospholipase C-mediated production of inositol 1,4,5-trisphosphate and causes sinusoidal oscillations in [Ca2+]i. Conversely, aromatic amino acid-induced CaR activation does not stimulate phospholipase C but engages an unidentified signaling mechanism that promotes transient oscillations in [Ca 2+]i. We show here that the [Ca2+]i oscillations stimulated by aromatic amino acids were selectively abolished by TRPC1 down-regulation using either a pool of small inhibitory RNAs (siRNAs) or two different individual siRNAs that targeted different coding regions of TRPC1. Furthermore, [Ca2+]i oscillations stimulated by aromatic amino acids were also abolished by inhibition of TRPC1 function with an antibody that binds the pore region of the channel. We also show that aromatic amino acid-stimulated [Ca2+]i oscillations can be prevented by protein kinase C (PKC) inhibitors or siRNA-mediated PKCα down-regulation and impaired by either calmodulin antagonists or by the expression of a dominant-negative calmodulin mutant. We propose a model for the generation of CaR-mediated transient [Ca2+]i oscillations that integrates its stimulation by aromatic amino acids with TRPC1 regulation by PKC and calmodulin.

Original languageEnglish (US)
Pages (from-to)38730-38737
Number of pages8
JournalJournal of Biological Chemistry
Volume281
Issue number50
DOIs
StatePublished - Dec 15 2006

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Calcium-Sensing Receptors
Aromatic Amino Acids
Cations
Calmodulin
Protein Kinase C
Type C Phospholipases
Down-Regulation
RNA
Inositol 1,4,5-Trisphosphate
Protein C Inhibitor
Protein Kinase Inhibitors
Small Interfering RNA
Chemical activation
Antibodies
Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calcium-sensing receptor. / Rey, Osvaldo; Young, Steven H.; Papazyan, Romeo; Shapiro, Mark S; Rozengurt, Enrique.

In: Journal of Biological Chemistry, Vol. 281, No. 50, 15.12.2006, p. 38730-38737.

Research output: Contribution to journalArticle

Rey, Osvaldo ; Young, Steven H. ; Papazyan, Romeo ; Shapiro, Mark S ; Rozengurt, Enrique. / Requirement of the TRPC1 cation channel in the generation of transient Ca2+ oscillations by the calcium-sensing receptor. In: Journal of Biological Chemistry. 2006 ; Vol. 281, No. 50. pp. 38730-38737.
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