Requirement of Skp1-Bub1 interaction for kinetochore-mediated activation of the spindle checkpoint

Katsumi Kitagawa, Rashid Abdulle, Parmil K. Bansal, Gerard Cagney, Stanley Fields, Philip Hieter

Research output: Contribution to journalArticlepeer-review

39 Scopus citations


The spindle checkpoint transiently prevents cell cycle progression of cells that have incurred errors or failed to complete steps during mitosis, including those involving kinetochore function. The molecular nature of the primary signal transmitted from defective kinetochores and how it is detected by the spindle checkpoint are unknown. We report biochemical evidence that Bub1, a component of the spindle checkpoint, associates with centromere (CEN) DNA via Skp1, a core kinetochore component in budding yeast. The Skp1's interaction with Bub1 is required for the mitotic delay induced by kinetochore tension defects, but not for the arrest induced by spindle depolymerization, kinetochore assembly defects, or Mps1 overexpression. We propose that the Skp1-Bub1 interaction is important for transmitting a signal to the spindle checkpoint pathway when insufficient tension is present at kinetochores.

Original languageEnglish (US)
Pages (from-to)1201-1213
Number of pages13
JournalMolecular Cell
Issue number5
StatePublished - May 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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