Abstract
[Nielsen et al., 2001: Curr Biol 11:529-533], based on studies in Drosophila, have proposed that β tubulin in axonemal microtubules must contain a specific acidic seven amino acid sequence in its carboxyl terminus. In mammals, the two βIV isotypes (βIVa and βIVb) contain that sequence. In order to test the application of this hypothesis to mammals, we have examined the expression of β tubulin isotypes in four different ciliated tissues (trachea, ependyma, uterine tube, and testis) using isotype-specific antibodies and indirect immunofluorescence. We find that βIV tubulin is present in all ciliated cell types examined, but so is β1 tubulin. Taken together with recent studies that show that β1 and βIV tubulin are both present in the cilia of vestibular hair cells, olfactory neurons, and nasal respiratory epithelial cells, we propose that both β1 tubulin and βIV tubulin may be required for axonemal structures in mammals.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 213-220 |
| Number of pages | 8 |
| Journal | Cell Motility and the Cytoskeleton |
| Volume | 55 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jul 1 2003 |
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Keywords
- Cilia
- Ependyma
- Fallopian tube
- Testis
- Trachea
- Tubulin isotypes
ASJC Scopus subject areas
- Cell Biology
Cite this
Requirement for the βI and βIV tubulin isotypes in mammalian cilia. / Jensen-Smith, Heather C.; Ludueña, Richard F.; Hallworth, Richard.
In: Cell Motility and the Cytoskeleton, Vol. 55, No. 3, 01.07.2003, p. 213-220.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Requirement for the βI and βIV tubulin isotypes in mammalian cilia
AU - Jensen-Smith, Heather C.
AU - Ludueña, Richard F.
AU - Hallworth, Richard
PY - 2003/7/1
Y1 - 2003/7/1
N2 - [Nielsen et al., 2001: Curr Biol 11:529-533], based on studies in Drosophila, have proposed that β tubulin in axonemal microtubules must contain a specific acidic seven amino acid sequence in its carboxyl terminus. In mammals, the two βIV isotypes (βIVa and βIVb) contain that sequence. In order to test the application of this hypothesis to mammals, we have examined the expression of β tubulin isotypes in four different ciliated tissues (trachea, ependyma, uterine tube, and testis) using isotype-specific antibodies and indirect immunofluorescence. We find that βIV tubulin is present in all ciliated cell types examined, but so is β1 tubulin. Taken together with recent studies that show that β1 and βIV tubulin are both present in the cilia of vestibular hair cells, olfactory neurons, and nasal respiratory epithelial cells, we propose that both β1 tubulin and βIV tubulin may be required for axonemal structures in mammals.
AB - [Nielsen et al., 2001: Curr Biol 11:529-533], based on studies in Drosophila, have proposed that β tubulin in axonemal microtubules must contain a specific acidic seven amino acid sequence in its carboxyl terminus. In mammals, the two βIV isotypes (βIVa and βIVb) contain that sequence. In order to test the application of this hypothesis to mammals, we have examined the expression of β tubulin isotypes in four different ciliated tissues (trachea, ependyma, uterine tube, and testis) using isotype-specific antibodies and indirect immunofluorescence. We find that βIV tubulin is present in all ciliated cell types examined, but so is β1 tubulin. Taken together with recent studies that show that β1 and βIV tubulin are both present in the cilia of vestibular hair cells, olfactory neurons, and nasal respiratory epithelial cells, we propose that both β1 tubulin and βIV tubulin may be required for axonemal structures in mammals.
KW - Cilia
KW - Ependyma
KW - Fallopian tube
KW - Testis
KW - Trachea
KW - Tubulin isotypes
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UR - http://www.scopus.com/inward/citedby.url?scp=0038786976&partnerID=8YFLogxK
U2 - 10.1002/cm.10122
DO - 10.1002/cm.10122
M3 - Article
C2 - 12789665
AN - SCOPUS:0038786976
VL - 55
SP - 213
EP - 220
JO - Cytoskeleton
JF - Cytoskeleton
SN - 1949-3584
IS - 3
ER -