Reproducibility of disc susceptibility tests with haemophilus influenzae

A. L. Barry; J. H. Jorgensen; D. J. Hardy

doi:10.1093/jac/27.3.295

Reproducibility of disc susceptibility tests with haemophilus influenzae

A. L. Barry, J. H. Jorgensen, D. J. Hardy

Pathology

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Triplicate susceptibility tests were performed in each of three laboratories with 45 strains of Haemophilus influenzae and a single lot of Haemophilus test medium (HTM) agar plates. Using clarithromycin, erythromycin and chloramphenicol discs, zone sizes varied over ranges averaging only 4.3-5.8 mm. Ampicillin zone sizes were more variable, especially when β-lactamase-producing strains were being tested, but the latter strains were consistently categorized as being resistant. A 94% interpretive consistency was observed with the two macrolides.

Original language	English (US)
Pages (from-to)	295-301
Number of pages	7
Journal	Journal of Antimicrobial Chemotherapy
Volume	27
Issue number	3
DOIs	https://doi.org/10.1093/jac/27.3.295
State	Published - Mar 1991

ASJC Scopus subject areas

Pharmacology
Microbiology (medical)
Infectious Diseases
Pharmacology (medical)

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title = "Reproducibility of disc susceptibility tests with haemophilus influenzae",

abstract = "Triplicate susceptibility tests were performed in each of three laboratories with 45 strains of Haemophilus influenzae and a single lot of Haemophilus test medium (HTM) agar plates. Using clarithromycin, erythromycin and chloramphenicol discs, zone sizes varied over ranges averaging only 4.3-5.8 mm. Ampicillin zone sizes were more variable, especially when β-lactamase-producing strains were being tested, but the latter strains were consistently categorized as being resistant. A 94% interpretive consistency was observed with the two macrolides.",

author = "Barry, {A. L.} and Jorgensen, {J. H.} and Hardy, {D. J.}",

note = "Funding Information: We thank Dr George L. Evans for his help in obtaining the media that were used in this evaluation and to Dr Richard A. Rode for his excellent statistical advice. We are also thankful to Abbott Laboratories for financial support. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "1991",

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AU - Jorgensen, J. H.

AU - Hardy, D. J.

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AB - Triplicate susceptibility tests were performed in each of three laboratories with 45 strains of Haemophilus influenzae and a single lot of Haemophilus test medium (HTM) agar plates. Using clarithromycin, erythromycin and chloramphenicol discs, zone sizes varied over ranges averaging only 4.3-5.8 mm. Ampicillin zone sizes were more variable, especially when β-lactamase-producing strains were being tested, but the latter strains were consistently categorized as being resistant. A 94% interpretive consistency was observed with the two macrolides.

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Reproducibility of disc susceptibility tests with haemophilus influenzae

Abstract

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