Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters

Kadosh David, Struhl Kevin

Research output: Contribution to journalArticlepeer-review

440 Scopus citations

Abstract

Sin3 and Rpd3 negatively regulate a diverse set of yeast genes. A mouse Sin3-related protein is a transcriptional corepressor, and a human Rpd3 homolog is a histone deacetylase. Here, we show that Sin3 and Rpd3 are specifically required for transcriptional repression by Ume6, a DNA-binding protein that regulates genes involved in meiosis. A short region of Ume6 is sufficient to repress transcription, and this repression domain mediates a two-hybrid and physical interaction with Sin3. Coimmunoprecipitation and two- hybrid experiments indicate that Sin3 and Rpd3 are associated in a complex distinct from TFIID and Pol II holoenzyme. Rpd3 is specifically required for repression by Sin3, and artificial recruitment of Rpd3 results in repression. These results suggest that repression by Ume6 involves recruitment of a Sin3- Rpd3 complex and targeted histone deacetylation.

Original languageEnglish (US)
Pages (from-to)365-371
Number of pages7
JournalCell
Volume89
Issue number3
DOIs
StatePublished - May 2 1997

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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