TY - JOUR
T1 - Replication of integrative data analysis for adipose tissue dysfunction, low-grade inflammation, postprandial responses and omics signatures in symptom-free adults
AU - Gallegos-Cabriales, Esther C.
AU - Rodriguez-Ayala, Ernesto
AU - Laviada-Molina, Hugo A.
AU - Nava-Gonzalez, Edna J.
AU - Salinas-Osornio, Rocío A.
AU - Orozco, Lorena
AU - Leal-Berumen, Irene
AU - Castillo-Pineda, Juan Carlos
AU - Gonzalez-Lopez, Laura
AU - Escudero-Lourdes, Claudia
AU - Cornejo-Barrera, Judith
AU - Escalante-Araiza, Fabiola
AU - Huerta-Avila, Eira E.
AU - Buenfil-Rello, Fatima A.
AU - Peschard, Vanessa Giselle
AU - Silva, Eliud
AU - Veloz-Garza, Rosa A.
AU - Martinez-Hernandez, Angelica
AU - Barajas-Olmos, Francisco M.
AU - Molina-Segui, Fernanda
AU - Gonzalez-Ramirez, Lucia
AU - Arjona-Villicaña, Ruy D.
AU - Hernandez-Escalante, Victor M.
AU - Gaytan-Saucedo, Janeth F.
AU - Vaquera, Zoila
AU - Acebo-Martinez, Monica
AU - Murillo-Ramirez, Areli
AU - Diaz-Tena, Sara P.
AU - Figueroa-Nuñez, Benigno
AU - Valencia-Rendon, Melesio E.
AU - Garzon-Zamora, Rafael
AU - Viveros-Paredes, Juan Manuel
AU - Valdovinos-Chavez, Salvador B.
AU - Comuzzie, Anthony G.
AU - Haack, Karin
AU - Thorsell, Ashley A.
AU - Han, Xianlin
AU - Cole, Shelley A.
AU - Bastarrachea, Raul A.
N1 - Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/12
Y1 - 2021/12
N2 - We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (<1) or high (>1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals through low-grade chronic subclinical inflammation (high sensitivity C-reactive protein (hsCRP)), whole body insulin sensitivity (Matsuda Index) and Metabolic Syndrome criteria in people with/without AT dysfunction. Molecular data directly measured from three tissues in a subset of participants allowed fine-scale multi-OMIC profiling of individual postprandial responses (RNA-seq in SKM and SQAT, miRNA from plasma exosomes and shotgun lipidomics in blood). Dynamic postprandial immunometabolic molecular endophenotypes were obtained to move towards a personalized, patient-defined medicine. This study offers an example of integrative translational research, which applies bench-to-bedside research to clinical medicine. Our F/P study design has the potential to characterize CV/immunometabolic early risk detection in support of precision medicine and discovery in SF individuals.
AB - We previously reported preliminary characterization of adipose tissue (AT) dysfunction through the adiponectin/leptin ratio (ALR) and fasting/postprandial (F/P) gene expression in subcutaneous (SQ) adipose tissue (AT) biopsies obtained from participants in the GEMM study, a precision medicine research project. Here we present integrative data replication of previous findings from an increased number of GEMM symptom-free (SF) adults (N = 124) to improve characterization of early biomarkers for cardiovascular (CV)/immunometabolic risk in SF adults with AT dysfunction. We achieved this goal by taking advantage of the rich set of GEMM F/P 5 h time course data and three tissue samples collected at the same time and frequency on each adult participant (F/P blood, biopsies of SQAT and skeletal muscle (SKM)). We classified them with the presence/absence of AT dysfunction: low (<1) or high (>1) ALR. We also examined the presence of metabolically healthy (MH)/unhealthy (MUH) individuals through low-grade chronic subclinical inflammation (high sensitivity C-reactive protein (hsCRP)), whole body insulin sensitivity (Matsuda Index) and Metabolic Syndrome criteria in people with/without AT dysfunction. Molecular data directly measured from three tissues in a subset of participants allowed fine-scale multi-OMIC profiling of individual postprandial responses (RNA-seq in SKM and SQAT, miRNA from plasma exosomes and shotgun lipidomics in blood). Dynamic postprandial immunometabolic molecular endophenotypes were obtained to move towards a personalized, patient-defined medicine. This study offers an example of integrative translational research, which applies bench-to-bedside research to clinical medicine. Our F/P study design has the potential to characterize CV/immunometabolic early risk detection in support of precision medicine and discovery in SF individuals.
KW - Adipose tissue dysfunction
KW - Fat and muscle tissue biopsies
KW - Low-grade chronic subclinical inflammation
KW - Metabolically healthy/unhealthy phenotype
KW - OMICs molecular signatures
KW - Postprandial inflammatory response
KW - Symptom-free volunteers
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U2 - 10.3390/biology10121342
DO - 10.3390/biology10121342
M3 - Article
C2 - 34943258
AN - SCOPUS:85121581174
SN - 2079-7737
VL - 10
JO - Biology
JF - Biology
IS - 12
M1 - 1342
ER -