Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses

Christian Griot, Andrew Pekosz, Richard Davidson, Kara Stillmock, Maarten Hoek, David Lukac, Dan Schmeidler, Ingenue Cobbinah, Francisco Gonzalez-Scarano, Neal Nathanson

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Abstract

The neuroinvasiveness of California serogroup bunyaviruses is determined by the ability of the virus to replicate in striated muscle after peripheral inoculation of mice. Neuroinvasiveness was mapped to the medium (M) RNA segment of the virus, which encodes the viral glycoproteins, when reassortants were made between La Crosse/original virus, a neuroinvasive isolate, and Tahyna-181/57 virus, a nonneuroinvasive clone. We have tested the murine muscle cell line C2C12 as a surrogate for myotropism and have found that there is a slight, but reproducible difference in the replication of virus clones bearing the M RNA segment of La Crosse/original virus compared to clones bearing the M RNA segment of Tahyna-181/57 virus, as determined by viral titer, antigen expression, and plaque formation.

Original languageEnglish (US)
Article number71308
Pages (from-to)399-403
Number of pages5
JournalVirology
Volume201
Issue number2
DOIs
StatePublished - Jun 1994

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ASJC Scopus subject areas

  • Virology

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Griot, C., Pekosz, A., Davidson, R., Stillmock, K., Hoek, M., Lukac, D., Schmeidler, D., Cobbinah, I., Gonzalez-Scarano, F., & Nathanson, N. (1994). Replication in Cultured C2C12 Muscle Cells Correlates with the Neuroinvasiveness of California Serogroup Bunyaviruses. Virology, 201(2), 399-403. [71308]. https://doi.org/10.1006/viro.1994.1308