Relocalization of STIM1 for activation of store-operated Ca2+ entry is determined by the depletion of subplasma membrane endoplasmic reticulum Ca2+ store

Ling Ong Hwei, Xibao Liu, Krasimira Tsaneva-Atanasova, Brij B. Singh, Bidhan C. Bandyopadhyay, William D. Swaim, James T. Russell, Ramanujan S. Hegde, Arthur Sherman, Indu S. Ambudkar

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

STIM1 (stromal interacting molecule 1), an endoplasmic reticulum (ER) protein that controls store-operated Ca2+ entry (SOCE), redistributes into punctae at the cell periphery after store depletion. This redistribution is suggested to have a causal role in activation of SOCE. However, whether peripheral STIM1 punctae that are involved in regulation of SOCE are determined by depletion of peripheral or more internal ER has not yet been demonstrated. Here we show that Ca2+ depletion in subplasma membrane ER is sufficient for peripheral redistribution of STIM1 and activation of SOCE. 1 μM thapsigargin (Tg) induced substantial depletion of intracellular Ca 2+ stores and rapidly activated SOCE. In comparison, 1 nM Tg induced slower, about 60-70% less Ca2+ depletion but similar SOCE. SOCE was confirmed by measuring ISOC in addition to Ca2+, Mn 2+, and Ba2+ entry. Importantly, 1 nM Tg caused redistribution of STIM1 only in the ER-plasma membrane junction, whereas 1 μM Tg caused a relatively global relocalization of STIM1 in the cell. During the time taken for STIM1 relocalization and SOCE activation, 1 nM Bodipy-fluorescein Tg primarily labeled the subplasma membrane region, whereas 1 μM Tg labeled the entire cell. The localization of Tg in the subplasma membrane region was associated with depletion of ER in this region and activation of SOCE. Together, these data suggest that peripheral STIM1 relocalization that is causal in regulation of SOCE is determined by the status of [Ca2+] in the ER in close proximity to the plasma membrane. Thus, the mechanism involved in regulation of SOCE is contained within the ER-plasma membrane junctional region.

Original languageEnglish (US)
Pages (from-to)12176-12185
Number of pages10
JournalJournal of Biological Chemistry
Volume282
Issue number16
DOIs
StatePublished - Apr 20 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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