Release of osteoclast activating factor by normal human peripheral blood leukocytes

Clarence L. Trummel, Gregory R. Mundy, Lawrence G. Raisz

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

A reliable method for obtaining osteoclast activating factor (OAF) in the culture medium of phytohemagglutinin-activated peripheral blood leukocytes is described. OAF was detected by its ability to stimulate resorption of fetal rat bone in organ culture. Most bone resorbing activity was released by activated leukocytes during the first 24 hours of culture, well before 3H-thymidine incorporation was increased. Cultures maintained for longer than 3 days showed a decrease in OAF activity. Stimulated leukocytes cultured in medium with as little as 0.01 per cent plasma showed increased 3H-thymidine incorporation and released as much OAF as leukocytes cultured with 20 per cent plasma. OAF release was stimulated by pokeweed mitogen and concanavalin A as well as by phytohemagglutinin.

Original languageEnglish (US)
Pages (from-to)1001-1007
Number of pages7
JournalThe Journal of Laboratory and Clinical Medicine
Volume85
Issue number6
StatePublished - Jun 1975
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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