Release from quiescence stimulates the expression of integrin α5β1 which regulates DNA synthesis in human fibrosarcoma HT1080 cells

Danhui Wang, Thomas M. Birkenmeier, Junhua Yang, Srinivas Venkateswarlu, Lisa Humphrey, Michael G. Brattain, Luzhe Sun

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

We show that integrin or, subunit expression is stimulated when human fibrosarcoma HT1080 cells are released from quiescence. The a, subunit mRNA level in quiescent HT1080 cells was increased 24 hr after their release by 10% fetal bovine serum-containing medium reaching a maximum of 2.5 fold on day 2. Similar levels of induction of cell-surface α5 subunit protein as well as β1 subunit protein were also observed. This resulted in a significant increase of cell attachment to fibronectin. The serum stimulation also increased α5 subunit promoter activity by twofold which was protein synthesis independent. Subsequent deletion of a, subunit promoter DNA showed that the cis-element responsible for the activation is located between -92 bp and the transcription start site. The promoter activity was not induced until 12 hr after the release. Comparison of the effect of a serum-free medium and a 10% fetal bovine serum-supplemented medium revealed that both the DNA synthesis and α5 subunit induction were independent of exogenous growth factors. The increased integrin α5β1 appears to function by reducing mitogenic activity since blockade of fibronectin binding to its receptor with a RGD peptide, a monoclonal anti-fibronectin antibody, or a monoclonal anti-α5 subunit antibody during the release from quiescence significantly stimulated DNA synthesis. On the other hand, stable overexpression of the α5 subunit resulted in decreased DNA synthesis.

Original languageEnglish (US)
Pages (from-to)499-508
Number of pages10
JournalJournal of Cellular Physiology
Volume164
Issue number3
DOIs
StatePublished - Sep 1995
Externally publishedYes

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Fibrosarcoma
Integrins
Fibronectins
DNA
Protein Subunits
Serum
Antibodies
Transcription Initiation Site
Serum-Free Culture Media
Anti-Idiotypic Antibodies
Intercellular Signaling Peptides and Proteins
Chemical activation
Monoclonal Antibodies
Cells
Messenger RNA
Proteins

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

Cite this

Release from quiescence stimulates the expression of integrin α5β1 which regulates DNA synthesis in human fibrosarcoma HT1080 cells. / Wang, Danhui; Birkenmeier, Thomas M.; Yang, Junhua; Venkateswarlu, Srinivas; Humphrey, Lisa; Brattain, Michael G.; Sun, Luzhe.

In: Journal of Cellular Physiology, Vol. 164, No. 3, 09.1995, p. 499-508.

Research output: Contribution to journalArticle

Wang, Danhui ; Birkenmeier, Thomas M. ; Yang, Junhua ; Venkateswarlu, Srinivas ; Humphrey, Lisa ; Brattain, Michael G. ; Sun, Luzhe. / Release from quiescence stimulates the expression of integrin α5β1 which regulates DNA synthesis in human fibrosarcoma HT1080 cells. In: Journal of Cellular Physiology. 1995 ; Vol. 164, No. 3. pp. 499-508.
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