Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor

Kenneth M Hargreaves, A. H. Costello, J. L. Joris

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.

Original languageEnglish (US)
Pages (from-to)476-482
Number of pages7
JournalNeuroendocrinology
Volume49
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

beta-Endorphin
Corticotropin-Releasing Hormone
Carrageenan
Hindlimb
Corticotrophs
Calcitonin Gene-Related Peptide
Thromboplastin
Bradykinin
Denervation
Substance P
Cultured Cells
Cell Culture Techniques
Calcium
Injections
Wounds and Injuries

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor. / Hargreaves, Kenneth M; Costello, A. H.; Joris, J. L.

In: Neuroendocrinology, Vol. 49, No. 5, 1989, p. 476-482.

Research output: Contribution to journalArticle

@article{223c73e361044946bc561dd83aeb2eba,
title = "Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor",
abstract = "In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.",
author = "Hargreaves, {Kenneth M} and Costello, {A. H.} and Joris, {J. L.}",
year = "1989",
language = "English (US)",
volume = "49",
pages = "476--482",
journal = "Neuroendocrinology",
issn = "0028-3835",
publisher = "S. Karger AG",
number = "5",

}

TY - JOUR

T1 - Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor

AU - Hargreaves, Kenneth M

AU - Costello, A. H.

AU - Joris, J. L.

PY - 1989

Y1 - 1989

N2 - In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.

AB - In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimb denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRF-like factor which stimulates iB-END release both in the denervated rat and cultured pituitary cells.

UR - http://www.scopus.com/inward/record.url?scp=0024544293&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024544293&partnerID=8YFLogxK

M3 - Article

C2 - 2524675

AN - SCOPUS:0024544293

VL - 49

SP - 476

EP - 482

JO - Neuroendocrinology

JF - Neuroendocrinology

SN - 0028-3835

IS - 5

ER -