Release from inflamed tissue of a substance with properties similar to corticotropin-releasing factor

Kenneth M. Hargreaves, Ann H. Costetio, Jean L. Joris

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


In response to stressors involving tissue injury, pituitary corticotroph secretion of immunoreactive beta-endorphin (iB-END) could be either due to release of hypothalamic factors such as corticotropin-releasing factor (CRF) or to release of a tissue factor from the periphery. In the present experiments, we investigated whether inflamed tissue releases a factor which evokes pituitary secretion of iB-END. In an initial experiment, rats with an inflamed hindpaw due to carrageenan injection had significantly greater levels of circulating iB-END as compared to rats with saline-injected paws. Removal of afferent input, by hindlimh denervation, failed to block the carrageenan-induced increase in iB-END levels. Subcutaneous perfusates were then collected from inflamed and control hindlimbs and applied to rat anterior pituitary cell cultures. Pituitary release of iB-END due to administration of perfusate from inflamed paws was significantly greater than iB-END release due to perfusate from saline-injected paws or to basal release. The releasing activity in the perfusates was blocked in calcium-free medium and was not due to a direct action of carrageenan, bradykinin, substance P or calcitonin gene-related peptide. The results indicate that inflamed tissue releases a CRf--like factor which stimulates iB-END release both in the denervaled rat and cultured pituitary cells.

Original languageEnglish (US)
Pages (from-to)476-482
Number of pages7
Issue number5
StatePublished - Jan 1 1989
Externally publishedYes


  • Beta-endorphin
  • Carrageenan
  • Corticotropin-releasing factor
  • Inflammation
  • Pituitary corticotroph

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology
  • Endocrine and Autonomic Systems
  • Cellular and Molecular Neuroscience


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