Relative photoreactivity of the pigment inclusions of the retinal pigment epithelium

Randolph D. Glickman, Alexander E. Dontsov, Mikhail A. Ostrovsky

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The cellular pigments of the retinal pigment epithelium are photoactive; that is, they promote free radical oxidative reactions when illuminated with visible or ultraviolet light. This activity is sufficient to cause photo-oxidation of several major cellular components such as antioxidants, dinucleotide cofactors, proteins and fatty acids. The present investigation determined the relative ability of melanin, lipofuscin, and melanolipofuscin granules isolated from human and bovine eyes to oxidize linoleic and docosahexaenoic acids, which are polyunsaturated fatty acids. The dark reactivity as well as the light-stimulated reactions were determined. All RPE pigment granules stimulated fatty acid oxidation when irradiated with the blue-green (488.1 and 514.5 nm) emission of the Argon-ion laser. Only lipofuscin, however, caused peroxidation of fatty acids in the dark. These findings not only suggest that accumulation of lipofuscin in the aging eye may contribute to increased photooxidative stress to the retina and RPE, but also that the photoactive RPE pigments might serve as endogenous photosensitizers for therapeutic applications.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
EditorsS.L. Jacques
Pages46-51
Number of pages6
Volume3254
DOIs
StatePublished - 1998
EventLaser-Tissue Interaction IX - San Jose, CA, United States
Duration: Jan 26 1998Jan 28 1998

Other

OtherLaser-Tissue Interaction IX
CountryUnited States
CitySan Jose, CA
Period1/26/981/28/98

Keywords

  • Lipofuscin
  • Melanin
  • Melanolipofuscin
  • Photooxidative stress
  • Polyunsaturated fatty acids
  • RPE

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

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  • Cite this

    Glickman, R. D., Dontsov, A. E., & Ostrovsky, M. A. (1998). Relative photoreactivity of the pigment inclusions of the retinal pigment epithelium. In S. L. Jacques (Ed.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 3254, pp. 46-51) https://doi.org/10.1117/12.308206