Regulator DegU is required for multicellular behavior in Lysinibacillus sphaericus

Yimin Hu, Quanxin Cai, Shen Tian, Yong Ge, Zhiming Yuan, Xiaomin Hu

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

DegS and DegU make up a two component system belonging to a class of signal transduction systems that play important roles in a broad range of bacterial responses to the environment. However, little study has been done to explore the physiological functions of DegS-DegU in mosquitocidal Lysinibacillus sphaericus. In this study, it was found that deletion of degU or degS-degU inhibited the swarming motility, biofilm formation, sporulation and binary toxin production through regulating the related genes, and phosphorylation was necessary for the functions of DegU. Based on the findings, a regulation network mediated by DegU was delineated. Both DegU-pi and Spo0A-pi positively regulates genes which are linked with the transition from stage Ⅱ to the end of the sporulation process and also influences the production of binary toxins via regulation on sigE. Both DegU-pi and Spo0A-pi negatively regulate abrB/sinR and influence the biofilm formation. DegU-pi can positively regulate the motility via the regulation on sigD. Whether the regulations are directly or indirectly need to be explored. Moreover, Spo0A-pi may indirectly regulate the swarming motility through negatively regulating DegU. It was concluded that DegU is a global transcriptional regulator on cell swarming motility, biofilm formation, sporulation and virulence in L. sphaericus.

Original languageEnglish (US)
Pages (from-to)177-187
Number of pages11
JournalResearch in Microbiology
Volume169
Issue number3
DOIs
StatePublished - Apr 2018
Externally publishedYes

Keywords

  • DegU
  • Lysinibacillus sphaericus
  • Mosquitocidal
  • Spo0A
  • Sporulation

ASJC Scopus subject areas

  • Molecular Biology
  • Microbiology

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