Regulation of type I collagen gene expression in bone

Barbara E. Kream, John R. Harrison, Paul H. Krebsbach, Zoran Bogdanovic, Antonio Bedalov, Dubravko Pavlin, Charles O. Woody, Stephen H. Clark, David Rowe, Alexander C. Lichtler

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

The regulation of COL 1A1 gene expression in bone was studied by measuring the activity of type I collagen promoter fusion genes (ColCAT) in permanently transfected osteoblastic cells and calvariae from transgenic animals. The basal activity of ColCAT fusion genes in transfected cells is mediated by DNA sequences between -3.5 to -2.3 kb while expression in vivo requires sequences between -2.3 and -1.7 kb. Parathyroid hormone, 1,25-dihydroxyvitamin D3 and interleukin-1 decrease the activity of ColCAT fusion genes in osteoblastic cells and transgenic calvariae. Because there may be differences between the expression of ColCAT fusion genes in cultured cells and intact bone, it will be important to compare data obtained from transfected cells with an in vivo model such as calvariae from transgenic mice.

Original languageEnglish (US)
Pages (from-to)261-264
Number of pages4
JournalConnective Tissue Research
Volume31
Issue number4
DOIs
StatePublished - Jan 1 1995

Keywords

  • Bone
  • COL1A1
  • Transgenic mice
  • Type 1 collagen

ASJC Scopus subject areas

  • Rheumatology
  • Biochemistry
  • Orthopedics and Sports Medicine
  • Molecular Biology
  • Cell Biology

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