Regulation of the norepinephrine transporter by chronic administration of antidepressants

Saloua Benmansour, Alfonso V. Altamirano, David J. Jones, Teresa A. Sanchez, Georgianna G Gould, Marie Christine Pardon, David A Morilak, Alan Frazer

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Background: Downregulation of serotonin transporter was observed previously after chronic treatment with selective serotonin reuptake inhibitors (SSRIs) but not selective norepinephrine reuptake inhibitors (NRIs). This study investigated if chronic treatment of rats with selective NRIs or SSRIs also affected the norepinephrine transporter (NET). Methods: Rats were treated for 3 to 6 weeks by osmotic minipumps with either the selective NRIs, desipramine, or the SSRI paroxetine. Results: [3H]nisoxetine binding sites as well as [3H]norepinephrine uptake were decreased in hippocampus and cortex after treatment with desipramine. By contrast, paroxetine-treated rats showed no alteration in either [3H]nisoxetine binding or [ 3H]norepinephrine uptake. NET messenger RNA levels in the locus coeruleus were unchanged by desipramine treatment. Conclusions: These results demonstrate that the marked decrease in NET density 1) is not a consequence of a decrease in gene expression; 2) was caused only by a selective NRI; and 3) was associated with a parallel decrease in norepinephrine uptake.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalBiological Psychiatry
Volume55
Issue number3
DOIs
StatePublished - Feb 1 2004

Fingerprint

Norepinephrine Plasma Membrane Transport Proteins
Antidepressive Agents
Norepinephrine
nisoxetine
Desipramine
Serotonin Uptake Inhibitors
Paroxetine
Serotonin Plasma Membrane Transport Proteins
Locus Coeruleus
Hippocampus
Down-Regulation
Binding Sites
Gene Expression
Messenger RNA

Keywords

  • Antidepressant
  • Desipramine
  • Nisoxetine binding
  • Norepinephrine transporter
  • Norepinephrine uptake
  • Paroxetine

ASJC Scopus subject areas

  • Biological Psychiatry

Cite this

Benmansour, S., Altamirano, A. V., Jones, D. J., Sanchez, T. A., Gould, G. G., Pardon, M. C., ... Frazer, A. (2004). Regulation of the norepinephrine transporter by chronic administration of antidepressants. Biological Psychiatry, 55(3), 313-316. https://doi.org/10.1016/S0006-3223(03)00676-0

Regulation of the norepinephrine transporter by chronic administration of antidepressants. / Benmansour, Saloua; Altamirano, Alfonso V.; Jones, David J.; Sanchez, Teresa A.; Gould, Georgianna G; Pardon, Marie Christine; Morilak, David A; Frazer, Alan.

In: Biological Psychiatry, Vol. 55, No. 3, 01.02.2004, p. 313-316.

Research output: Contribution to journalArticle

Benmansour S, Altamirano AV, Jones DJ, Sanchez TA, Gould GG, Pardon MC et al. Regulation of the norepinephrine transporter by chronic administration of antidepressants. Biological Psychiatry. 2004 Feb 1;55(3):313-316. https://doi.org/10.1016/S0006-3223(03)00676-0
Benmansour, Saloua ; Altamirano, Alfonso V. ; Jones, David J. ; Sanchez, Teresa A. ; Gould, Georgianna G ; Pardon, Marie Christine ; Morilak, David A ; Frazer, Alan. / Regulation of the norepinephrine transporter by chronic administration of antidepressants. In: Biological Psychiatry. 2004 ; Vol. 55, No. 3. pp. 313-316.
@article{8b96761c90bb41bc898f2444f6c22f8c,
title = "Regulation of the norepinephrine transporter by chronic administration of antidepressants",
abstract = "Background: Downregulation of serotonin transporter was observed previously after chronic treatment with selective serotonin reuptake inhibitors (SSRIs) but not selective norepinephrine reuptake inhibitors (NRIs). This study investigated if chronic treatment of rats with selective NRIs or SSRIs also affected the norepinephrine transporter (NET). Methods: Rats were treated for 3 to 6 weeks by osmotic minipumps with either the selective NRIs, desipramine, or the SSRI paroxetine. Results: [3H]nisoxetine binding sites as well as [3H]norepinephrine uptake were decreased in hippocampus and cortex after treatment with desipramine. By contrast, paroxetine-treated rats showed no alteration in either [3H]nisoxetine binding or [ 3H]norepinephrine uptake. NET messenger RNA levels in the locus coeruleus were unchanged by desipramine treatment. Conclusions: These results demonstrate that the marked decrease in NET density 1) is not a consequence of a decrease in gene expression; 2) was caused only by a selective NRI; and 3) was associated with a parallel decrease in norepinephrine uptake.",
keywords = "Antidepressant, Desipramine, Nisoxetine binding, Norepinephrine transporter, Norepinephrine uptake, Paroxetine",
author = "Saloua Benmansour and Altamirano, {Alfonso V.} and Jones, {David J.} and Sanchez, {Teresa A.} and Gould, {Georgianna G} and Pardon, {Marie Christine} and Morilak, {David A} and Alan Frazer",
year = "2004",
month = "2",
day = "1",
doi = "10.1016/S0006-3223(03)00676-0",
language = "English (US)",
volume = "55",
pages = "313--316",
journal = "Biological Psychiatry",
issn = "0006-3223",
publisher = "Elsevier USA",
number = "3",

}

TY - JOUR

T1 - Regulation of the norepinephrine transporter by chronic administration of antidepressants

AU - Benmansour, Saloua

AU - Altamirano, Alfonso V.

AU - Jones, David J.

AU - Sanchez, Teresa A.

AU - Gould, Georgianna G

AU - Pardon, Marie Christine

AU - Morilak, David A

AU - Frazer, Alan

PY - 2004/2/1

Y1 - 2004/2/1

N2 - Background: Downregulation of serotonin transporter was observed previously after chronic treatment with selective serotonin reuptake inhibitors (SSRIs) but not selective norepinephrine reuptake inhibitors (NRIs). This study investigated if chronic treatment of rats with selective NRIs or SSRIs also affected the norepinephrine transporter (NET). Methods: Rats were treated for 3 to 6 weeks by osmotic minipumps with either the selective NRIs, desipramine, or the SSRI paroxetine. Results: [3H]nisoxetine binding sites as well as [3H]norepinephrine uptake were decreased in hippocampus and cortex after treatment with desipramine. By contrast, paroxetine-treated rats showed no alteration in either [3H]nisoxetine binding or [ 3H]norepinephrine uptake. NET messenger RNA levels in the locus coeruleus were unchanged by desipramine treatment. Conclusions: These results demonstrate that the marked decrease in NET density 1) is not a consequence of a decrease in gene expression; 2) was caused only by a selective NRI; and 3) was associated with a parallel decrease in norepinephrine uptake.

AB - Background: Downregulation of serotonin transporter was observed previously after chronic treatment with selective serotonin reuptake inhibitors (SSRIs) but not selective norepinephrine reuptake inhibitors (NRIs). This study investigated if chronic treatment of rats with selective NRIs or SSRIs also affected the norepinephrine transporter (NET). Methods: Rats were treated for 3 to 6 weeks by osmotic minipumps with either the selective NRIs, desipramine, or the SSRI paroxetine. Results: [3H]nisoxetine binding sites as well as [3H]norepinephrine uptake were decreased in hippocampus and cortex after treatment with desipramine. By contrast, paroxetine-treated rats showed no alteration in either [3H]nisoxetine binding or [ 3H]norepinephrine uptake. NET messenger RNA levels in the locus coeruleus were unchanged by desipramine treatment. Conclusions: These results demonstrate that the marked decrease in NET density 1) is not a consequence of a decrease in gene expression; 2) was caused only by a selective NRI; and 3) was associated with a parallel decrease in norepinephrine uptake.

KW - Antidepressant

KW - Desipramine

KW - Nisoxetine binding

KW - Norepinephrine transporter

KW - Norepinephrine uptake

KW - Paroxetine

UR - http://www.scopus.com/inward/record.url?scp=1642564355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642564355&partnerID=8YFLogxK

U2 - 10.1016/S0006-3223(03)00676-0

DO - 10.1016/S0006-3223(03)00676-0

M3 - Article

C2 - 14744474

AN - SCOPUS:1642564355

VL - 55

SP - 313

EP - 316

JO - Biological Psychiatry

JF - Biological Psychiatry

SN - 0006-3223

IS - 3

ER -