Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH

Albert Dee, Grace Carlson, Cydnie Smith, Bettie Sue Masters, Michael R. Waterman

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

Primary, monolayer cultures of bovine adrenocortical cells have been utilized to demonstrate that ACTH regulates the synthesis and thus the activity of NADPH-cytochrome P-450 reductase. The temporal pattern of induction correlates well with that previously reported for cytochromes P-45017α and P-450C21, microsomal steroid hydroxylases serviced by P-450 reductase. Results of in vitro translation studies suggest that ACTH regulates P-450 reductase synthesis at the transcriptional level and show that the adrenocortical enzyme is synthesized as the mature form. The action of ACTH to induce P-450 reductase synthesis in the adrenal cortex can be mimicked by dibutyryl cAMP, but not by phenobarbital which induces P-450 reductase synthesis in liver.

Original languageEnglish (US)
Pages (from-to)650-656
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume128
Issue number2
DOIs
StatePublished - Apr 30 1985

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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