TY - JOUR
T1 - Regulation of synthesis and activity of bovine adrenocortical NADPH-cytochrome P-450 reductase by ACTH
AU - Dee, Albert
AU - Carlson, Grace
AU - Smith, Cydnie
AU - Masters, Bettie Sue
AU - Waterman, Michael R.
PY - 1985/4/30
Y1 - 1985/4/30
N2 - Primary, monolayer cultures of bovine adrenocortical cells have been utilized to demonstrate that ACTH regulates the synthesis and thus the activity of NADPH-cytochrome P-450 reductase. The temporal pattern of induction correlates well with that previously reported for cytochromes P-45017α and P-450C21, microsomal steroid hydroxylases serviced by P-450 reductase. Results of in vitro translation studies suggest that ACTH regulates P-450 reductase synthesis at the transcriptional level and show that the adrenocortical enzyme is synthesized as the mature form. The action of ACTH to induce P-450 reductase synthesis in the adrenal cortex can be mimicked by dibutyryl cAMP, but not by phenobarbital which induces P-450 reductase synthesis in liver.
AB - Primary, monolayer cultures of bovine adrenocortical cells have been utilized to demonstrate that ACTH regulates the synthesis and thus the activity of NADPH-cytochrome P-450 reductase. The temporal pattern of induction correlates well with that previously reported for cytochromes P-45017α and P-450C21, microsomal steroid hydroxylases serviced by P-450 reductase. Results of in vitro translation studies suggest that ACTH regulates P-450 reductase synthesis at the transcriptional level and show that the adrenocortical enzyme is synthesized as the mature form. The action of ACTH to induce P-450 reductase synthesis in the adrenal cortex can be mimicked by dibutyryl cAMP, but not by phenobarbital which induces P-450 reductase synthesis in liver.
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U2 - 10.1016/0006-291X(85)90095-6
DO - 10.1016/0006-291X(85)90095-6
M3 - Article
C2 - 2986621
AN - SCOPUS:0022258935
SN - 0006-291X
VL - 128
SP - 650
EP - 656
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -