Regulation of steroid 17αhydroxylase in adrenocortical cells in culture

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3 Scopus citations

Abstract

The regulation of steroid 17αhydroxylase in human and bovine adrenocortical cells in culture is reviewed. It is shown that (i) the long-term growth and cloning of normal human fetal adrenocortical cells in culture is feasible; (ii) the phorbol ester 12-O-tetradecanoyl phorbol 13-acetate (TPA) is an effective mitogen in both clonal cultures and non-clonal early cultures of human adrenocortical cells, and shows a selective action in promoting adrenocortical cell growth and inhibiting fibroblast growth; (iii) the key steroidogenic enzymes, 17αhydroxylase and 3βhydroxysteroid dehydrogenase (3βHSD), are under dual regulation by the cyclic AMP and protein kinase C second messenger systems; (iv) for 17αhydroxylase, this dual regulation is mediated by changes in 17αhydroxylase mRNA levels; (v) bovine adrenocortical cells can be transfected with SV40 T antigen, producing lines with elevated differentiated functions, including stabilized high expression of 17αhydroxylase; (vi) human adrenocortical cells can also be transfected with SV40 large T antigen, giving rise to functional cell lines which may be useful in future studies of 17αhydroxylase regulation.

Original languageEnglish (US)
Pages (from-to)159-182
Number of pages24
JournalEndocrine Research
Volume15
Issue number1-2
DOIs
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • Endocrinology

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