Regulation of Rad51 function by phosphorylation

Sonja Flott, Youngho Kwon, Ying Zhang Pigli, Phoebe A. Rice, Patrick Sung, Stephen P. Jackson

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Rad51 is a key enzyme involved in DNA double-strand break repair by homologous recombination. Here, we show that in response to DNA damage, budding yeast Rad51 is phosphorylated on Ser 192 in a manner that is primarily mediated by the DNA-damage-responsive protein kinase Mec1. We show that mutating Rad51 Ser 192 to Ala or Glu confers hypersensitivity to DNA damage and homologous-recombination defects. Furthermore, biochemical analyses indicate that Ser 192 is required for Rad51 adenosine triphosphate hydrolysis and DNA-binding activity in vitro, whereas mutation of Ser 192 does not interfere with Rad51 multimer formation. These data suggest a model in which Mec1-mediated phosphorylation of Rad51 Ser 192 in response to DNA damage controls Rad51 activity and DNA repair by homologous recombination.

Original languageEnglish (US)
Pages (from-to)833-839
Number of pages7
JournalEMBO Reports
Volume12
Issue number8
DOIs
StatePublished - Aug 2011
Externally publishedYes

Keywords

  • DNA repair
  • Mec1
  • Rad51
  • homologous recombination
  • phosphorylation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics

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    Flott, S., Kwon, Y., Pigli, Y. Z., Rice, P. A., Sung, P., & Jackson, S. P. (2011). Regulation of Rad51 function by phosphorylation. EMBO Reports, 12(8), 833-839. https://doi.org/10.1038/embor.2011.127