Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase

Anupama Gururaj, Christopher J. Barnes, Ratna K. Vadlamudi, Rakesh Kumar

Research output: Contribution to journalArticle

64 Scopus citations

Abstract

We have identified a novel mechanism of cross-talk between cell signaling and metabolic pathways, whereby the signaling kinase p21-activated kinase 1 (Pak1) binds to, phosphorylates and enhances the enzymatic activity of phosphoglucomutase 1 (PGM), an important regulatory enzyme in cellular glucose utilization and energy homeostasis. Pak1 and PGM were colocalized in model cell systems and showed functional interactions in a physiological setting. Strong direct interaction of PGM with Pak1 but not Pak2, Pak3, or Pak4 was observed. PGM binding was within 75-149 amino acids (aa) of Pak1, while Pak1 binding to PGM was in the N-terminal 96aa. Pak1-mediated phosphorylation of PGM selectively on threonine 466 significantly increased PGM enzymatic activity and could be blocked by transfection with a dominant-negative Pak1 expression vector and by Pak1-specific small inhibitory RNA. Stable transfection of PGM into PGM-deficient K-562 leukemia cells further demonstrated the role of Pak1 in regulating PGM activity. The results presented here provide new evidence that the cell signaling kinase Pak1 is a novel regulator of glucose metabolism through its phosphorylation and regulation of PGM activity. These findings suggest a new mechanism whereby growth factor signaling may coordinately integrate metabolic regulation with established signaling functions of cell cycle regulation and cell growth.

Original languageEnglish (US)
Pages (from-to)8118-8127
Number of pages10
JournalOncogene
Volume23
Issue number49
DOIs
StatePublished - Oct 21 2004
Externally publishedYes

Keywords

  • Cancer metabolism
  • Glycolysis
  • PGM
  • Signaling

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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    Gururaj, A., Barnes, C. J., Vadlamudi, R. K., & Kumar, R. (2004). Regulation of phosphoglucomutase 1 phosphorylation and activity by a signaling kinase. Oncogene, 23(49), 8118-8127. https://doi.org/10.1038/sj.onc.1207969