Regulation of Na+/K+ ATPase transport velocity by RNA editing

Claudia Colina, Juan Pablo Palavicini, Deepa Srikumar, Miguel Holmgren, Joshua J.C. Rosenthal

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Because firing properties and metabolic rates vary widely, neurons require different transport rates from their Na+/K+ pumps in order to maintain ion homeostasis. In this study we show that Na+/K+ pump activity is tightly regulated by a novel process, RNA editing. Three codons within the squid Na+/K+ ATPase gene can be recoded at the RNA level, and the efficiency of conversion for each varies dramatically, and independently, between tissues. At one site, a highly conserved isoleucine in the seventh transmembrane span can be converted to a valine, a change that shifts the pump's intrinsic voltage dependence. Mechanistically, the removal of a single methyl group specifically targets the process of Na+ release to the extracellular solution, causing a higher turnover rate at the resting membrane potential.

Original languageEnglish (US)
Article numbere1000540
JournalPLoS biology
Volume8
Issue number11
DOIs
StatePublished - Nov 2010
Externally publishedYes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Agricultural and Biological Sciences(all)

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    Colina, C., Palavicini, J. P., Srikumar, D., Holmgren, M., & Rosenthal, J. J. C. (2010). Regulation of Na+/K+ ATPase transport velocity by RNA editing. PLoS biology, 8(11), [e1000540]. https://doi.org/10.1371/journal.pbio.1000540