Regulation of macrophage colony-stimulating factor in liver fat-storing cells by peptide growth factors

M. Pinzani, H. E. Abboud, L. Gesualdo, S. L. Abboud

Research output: Contribution to journalArticle

67 Scopus citations

Abstract

Macrophage colony-stimulating factor (M-CSF) selectively promotes mononuclear phagocyte survival, proliferation, and differentiation. The production of this factor within the liver may be necessary to support the relatively long-term survival of circulating monocytes as they migrate into tissues and differentiate into macrophages. We studied the constitutive expression and the effects of platelet-derived growth factor (PDGF), basic fibroblast growth factor (bFGF), and epidermal growth factor (EGF) on M-CSF mRNA levels and secretion of M-CSF in murine liver fat-storing cells (FSC), vascular pericytes likely involved in the development of liver fibrosis. By Northern analysis, using a murine M-CSF cDNA, FSC constitutively express two major transcripts of 4.4 and 2.2 kb, similar to those detected in mouse L cells, used as a control. Exposure to 10 ng/ml PDGF or bFGF increased M-CSF mRNA levels. Peak effects were observed at 3 and 6 h for PDGF and bFGF, respectively, returning to baseline levels by 12 h. Under basal conditions, detectable amounts of M-CSF, measured by radioimmunoassay, were found in cell supernatants conditioned for 8 and 24 h. PDGF and bFGF markedly stimulated the release of M-CSF as early as 8 h, an effect persisting for at least 24 h. These findings suggest that liver FSC release M-CSF upon stimulation by PDGF and bFGF and may contribute to the activation of resident or infiltrating cells in inflammatory liver diseases.

Original languageEnglish (US)
Pages (from-to)C876-C881
JournalAmerican Journal of Physiology - Cell Physiology
Volume262
Issue number4 31-4
StatePublished - Jan 1 1992

Keywords

  • basic fibroblast growth factor
  • platelet-derived growth factor

ASJC Scopus subject areas

  • Physiology
  • Cell Biology

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