Regulation of integrin gene expression by substrate adherence

D. Chen, V. Magnuson, S. Hill, C. Arnaud, B. Steffensen, R. J. Klebe

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Under substrate adherent conditions, integrin gene expression can be regulated by transforming growth factor-β, interleukin-1β, and prostaglandins. This report demonstrates a new mechanism that can differentially control the expression of several integrins. When MG-63 osteosarcoma cells are maintained in suspension, up-regulation of several integrin α-subunits takes place. Within as little as 4 h, the mRNA levels for both the α2- and α4-subunits are increased 4- and 6-fold, respectively. It was found that mRNA levels for the α2-, α4-, and α(v)- subunits were markedly increased in several differentiated cell lines under non-adherent conditions; however, cells that did not express a given integrin under substrate adherent conditions also did not express this integrin when maintained in suspension. The α5-subunit did not up-regulate during suspension growth. By immunocytochemistry, changes in integrin mRNA levels were confirmed at the protein level. Both cytochalasin B and a phorbol ester were found to induce the expression of the α2-subunit, but not the α4- and α5-subunits, in a dose-dependent fashion. Many investigators have documented changes in gene expression that result from changes in 'cell shape.' These phenomena may result from up-regulation of integrin gene expression induced by the lack of substrate adherence.

Original languageEnglish (US)
Pages (from-to)23502-23506
Number of pages5
JournalJournal of Biological Chemistry
Volume267
Issue number33
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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