Regulation of immune function by calorie restriction and cyclophosphamide treatment in lupus-prone NZB/NZW F1 mice

Dongxu Sun, Aparna Krishnan, Jianrong Su, Richard Lawrence, Khaliquz Zaman, Gabriel Fernandes

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex antibodies. CTX and CR prevented the increase in and activation of B cells, the decline in CD8+ T cells, and maintained a higher proportion of naïve CD4+ and CD8+ cells. MHC class I antigen and LFA-1 expression on CD8+ T cells and MHC class II antigen on B cells were also decreased. AL/CTX and CR prevented the increase in production of IL-10 and up-regulated IL-2 production in T cells ex vivo. We concluded that both CR and CTX can delay the onset of autoimmune disease, in part by maintaining higher numbers of naïve T cells and the immune responsiveness of T cells and decreasing the proportion of B cells.

Original languageEnglish (US)
Pages (from-to)54-65
Number of pages12
JournalCellular Immunology
Volume228
Issue number1
DOIs
StatePublished - Mar 2004

Fingerprint

Cyclophosphamide
T-Lymphocytes
B-Lymphocytes
Lymphocyte Function-Associated Antigen-1
Histocompatibility Antigens Class I
Lupus Nephritis
Histocompatibility Antigens Class II
Antigen-Antibody Complex
Proteinuria
Interleukin-10
Histones
Autoimmune Diseases
Interleukin-2
Antibodies
Serum

Keywords

  • Calorie restriction
  • Cyclophosphamide
  • Cytokines
  • SLE
  • T cells

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

Regulation of immune function by calorie restriction and cyclophosphamide treatment in lupus-prone NZB/NZW F1 mice. / Sun, Dongxu; Krishnan, Aparna; Su, Jianrong; Lawrence, Richard; Zaman, Khaliquz; Fernandes, Gabriel.

In: Cellular Immunology, Vol. 228, No. 1, 03.2004, p. 54-65.

Research output: Contribution to journalArticle

Sun, Dongxu ; Krishnan, Aparna ; Su, Jianrong ; Lawrence, Richard ; Zaman, Khaliquz ; Fernandes, Gabriel. / Regulation of immune function by calorie restriction and cyclophosphamide treatment in lupus-prone NZB/NZW F1 mice. In: Cellular Immunology. 2004 ; Vol. 228, No. 1. pp. 54-65.
@article{fb992923f68e4aa0a0e527d4c2c6c986,
title = "Regulation of immune function by calorie restriction and cyclophosphamide treatment in lupus-prone NZB/NZW F1 mice",
abstract = "We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex antibodies. CTX and CR prevented the increase in and activation of B cells, the decline in CD8+ T cells, and maintained a higher proportion of na{\"i}ve CD4+ and CD8+ cells. MHC class I antigen and LFA-1 expression on CD8+ T cells and MHC class II antigen on B cells were also decreased. AL/CTX and CR prevented the increase in production of IL-10 and up-regulated IL-2 production in T cells ex vivo. We concluded that both CR and CTX can delay the onset of autoimmune disease, in part by maintaining higher numbers of na{\"i}ve T cells and the immune responsiveness of T cells and decreasing the proportion of B cells.",
keywords = "Calorie restriction, Cyclophosphamide, Cytokines, SLE, T cells",
author = "Dongxu Sun and Aparna Krishnan and Jianrong Su and Richard Lawrence and Khaliquz Zaman and Gabriel Fernandes",
year = "2004",
month = "3",
doi = "10.1016/j.cellimm.2004.04.001",
language = "English (US)",
volume = "228",
pages = "54--65",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Regulation of immune function by calorie restriction and cyclophosphamide treatment in lupus-prone NZB/NZW F1 mice

AU - Sun, Dongxu

AU - Krishnan, Aparna

AU - Su, Jianrong

AU - Lawrence, Richard

AU - Zaman, Khaliquz

AU - Fernandes, Gabriel

PY - 2004/3

Y1 - 2004/3

N2 - We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex antibodies. CTX and CR prevented the increase in and activation of B cells, the decline in CD8+ T cells, and maintained a higher proportion of naïve CD4+ and CD8+ cells. MHC class I antigen and LFA-1 expression on CD8+ T cells and MHC class II antigen on B cells were also decreased. AL/CTX and CR prevented the increase in production of IL-10 and up-regulated IL-2 production in T cells ex vivo. We concluded that both CR and CTX can delay the onset of autoimmune disease, in part by maintaining higher numbers of naïve T cells and the immune responsiveness of T cells and decreasing the proportion of B cells.

AB - We compared the effects of calorie restriction (CR) and cyclophosphamide (CTX) on the progression of lupus nephritis and immunological changes in NZB/NZW F1 mice. Ad libitum (AL)/CTX and CR delayed onset of proteinuria and significantly decreased serum levels of anti-dsDNA, anti-histone, and circulating immune complex antibodies. CTX and CR prevented the increase in and activation of B cells, the decline in CD8+ T cells, and maintained a higher proportion of naïve CD4+ and CD8+ cells. MHC class I antigen and LFA-1 expression on CD8+ T cells and MHC class II antigen on B cells were also decreased. AL/CTX and CR prevented the increase in production of IL-10 and up-regulated IL-2 production in T cells ex vivo. We concluded that both CR and CTX can delay the onset of autoimmune disease, in part by maintaining higher numbers of naïve T cells and the immune responsiveness of T cells and decreasing the proportion of B cells.

KW - Calorie restriction

KW - Cyclophosphamide

KW - Cytokines

KW - SLE

KW - T cells

UR - http://www.scopus.com/inward/record.url?scp=2942618696&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942618696&partnerID=8YFLogxK

U2 - 10.1016/j.cellimm.2004.04.001

DO - 10.1016/j.cellimm.2004.04.001

M3 - Article

C2 - 15203320

AN - SCOPUS:2942618696

VL - 228

SP - 54

EP - 65

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -