Regulation of glomerular endothelial cell proteoglycans by glucose

Tae Sun Ha, Senthil Duraisamy, Jennifer L. Faulkner, Balakuntalam S Kasinath

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The presence of heparan sulfate proteoglycan (HSPG) in anionic sites in the lamina rara interna of glomerular basement membrane suggests that the proteoglycan may be deposited by the glomerular endothelial cells (GEndo). We have previously demonstrated that bovine GEndo in vitro synthesize perlecan, a species of glomerular basement membrane HSPG. In this study we examined whether high glucose medium regulates the GEndo metabolism of glycopeptides including perlecan. Metabolic labeling of glycoconjugates with 35S-SO 4, sequential ion exchange and Sepharose CL-4B chromatography of labeled glycoconjugates, and northern analysis were performed. Incubation of GEndo for 8 to 14 weeks (but not for 1-2 weeks) in medium containing 30 mM glucose resulted in nearly 50% reduction in the synthesis of cell layer and medium 35SO4-labeled low anionic glycoproteins and proteoglycans, including that of basement membrane HSPG (Kav 0.42) compared to GEndo grown in 5 mM glucose medium; no changes in anionic charge density or hydrodynamic size of proteoglycans were noted. Northern analysis demonstrated that the mRNA abundance of perlecan was reduced by 47% in cells incubated with 30 mM glucose. Our data suggest that high glucose medium reduces the GEndo synthesis of perlecan by regulating its gene expression. Reduced synthesis of perlecan by GEndo may contribute to proteinuria seen in diabetic nephropathy.

Original languageEnglish (US)
Pages (from-to)245-252
Number of pages8
JournalJournal of Korean Medical Science
Volume19
Issue number2
StatePublished - Apr 2004

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Proteoglycans
Endothelial Cells
Glucose
Heparan Sulfate Proteoglycans
Glomerular Basement Membrane
Glycoconjugates
Glycopeptides
Ion Exchange
Diabetic Nephropathies
Hydrodynamics
Proteinuria
Basement Membrane
Chromatography
Glycoproteins
perlecan
Gene Expression
Messenger RNA

Keywords

  • Diabetes Mellitus
  • Diabetic Nephropathies
  • Endothelium
  • Heparan Sulfate Proteoglycan
  • Kidney Glomerulus
  • Proteinuria

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ha, T. S., Duraisamy, S., Faulkner, J. L., & Kasinath, B. S. (2004). Regulation of glomerular endothelial cell proteoglycans by glucose. Journal of Korean Medical Science, 19(2), 245-252.

Regulation of glomerular endothelial cell proteoglycans by glucose. / Ha, Tae Sun; Duraisamy, Senthil; Faulkner, Jennifer L.; Kasinath, Balakuntalam S.

In: Journal of Korean Medical Science, Vol. 19, No. 2, 04.2004, p. 245-252.

Research output: Contribution to journalArticle

Ha, TS, Duraisamy, S, Faulkner, JL & Kasinath, BS 2004, 'Regulation of glomerular endothelial cell proteoglycans by glucose', Journal of Korean Medical Science, vol. 19, no. 2, pp. 245-252.
Ha TS, Duraisamy S, Faulkner JL, Kasinath BS. Regulation of glomerular endothelial cell proteoglycans by glucose. Journal of Korean Medical Science. 2004 Apr;19(2):245-252.
Ha, Tae Sun ; Duraisamy, Senthil ; Faulkner, Jennifer L. ; Kasinath, Balakuntalam S. / Regulation of glomerular endothelial cell proteoglycans by glucose. In: Journal of Korean Medical Science. 2004 ; Vol. 19, No. 2. pp. 245-252.
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