Regulation of extracellular matrix vesicles via rapid responses to steroid hormones during endochondral bone formation

Niels Asmussen, Zhao Lin, Michael J. McClure, Zvi Schwartz, Barbara D. Boyan

Research output: Contribution to journalArticle

Abstract

Endochondral bone formation is a precise and highly ordered process whose exact regulatory framework is still being elucidated. Multiple regulatory pathways are known to be involved. In some cases, regulation impacts gene expression, resulting in changes in chondrocyte phenotypic expression and extracellular matrix synthesis. Rapid regulatory mechanisms are also involved, resulting in release of enzymes, factors and micro RNAs stored in extracellular matrisomes called matrix vesicles. Vitamin D metabolites modulate endochondral development via both genomic and rapid membrane-associated signaling pathways. 1α,25-dihydroxyvitamin D3 [1α,25(OH)2D3] acts through the vitamin D receptor (VDR) and a membrane associated receptor, protein disulfide isomerase A3 (PDIA3). 24R,25-dihydroxyvitamin D3 [24R,25(OH)2D3] affects primarily chondrocytes in the resting zone (RC) of the growth plate, whereas 1α,25(OH)2D3 affects cells in the prehypertrophic and upper hypertrophic cell zones (GC). This includes genomically directing the cells to produce matrix vesicles with zone specific characteristics. In addition, vitamin D metabolites produced by the cells interact directly with the matrix vesicle membrane via rapid signal transduction pathways, modulating their activity in the matrix. The matrix vesicle payload is able to rapidly impact the extracellular matrix via matrix processing enzymes as well as providing a feedback mechanism to the cells themselves via the contained micro RNAs.

Original languageEnglish (US)
JournalSteroids
DOIs
StateAccepted/In press - Jan 1 2018

Fingerprint

Steroid hormones
Osteogenesis
Extracellular Matrix
Bone
Steroids
Hormones
Metabolites
Membranes
MicroRNAs
Vitamin D
Protein Disulfide-Isomerases
Signal transduction
Chondrocytes
Calcitriol Receptors
Calcitriol
Enzymes
Gene expression
Growth Plate
Feedback
Gene Expression Regulation

Keywords

  • Chondrocyte cell membrane
  • Exosomes
  • Growth plate cartilage
  • Matrisomes
  • Matrix vesicles
  • MiRNA

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology
  • Pharmacology
  • Clinical Biochemistry
  • Organic Chemistry

Cite this

Regulation of extracellular matrix vesicles via rapid responses to steroid hormones during endochondral bone formation. / Asmussen, Niels; Lin, Zhao; McClure, Michael J.; Schwartz, Zvi; Boyan, Barbara D.

In: Steroids, 01.01.2018.

Research output: Contribution to journalArticle

Asmussen, Niels ; Lin, Zhao ; McClure, Michael J. ; Schwartz, Zvi ; Boyan, Barbara D. / Regulation of extracellular matrix vesicles via rapid responses to steroid hormones during endochondral bone formation. In: Steroids. 2018.
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