Regulation of expression of the sensory neuron-specific sodium channel SNS in inflammatory and neuropathic pain

Kenji Okuse, Sandra R. Chaplan, Stephen B. McMahon, Z. David Luo, Nigel A. Calcutt, Brian P. Scott, Armen N Akopian, John N. Wood

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Increased voltage-gated sodium channel activity may contribute to the hyperexcitability of sensory neurons in inflammatory and neuropathic pain states. We examined the levels of the transcript encoding the tetrodotoxin- resistant sodium channel SNS in dorsal root ganglion neurons in a range of inflammatory and neuropathic pain models in the rat. Local Freund's adjuvant or systemic nerve growth factor-induced inflammation did not substantially alter the total levels of SNS mRNA. When NGF-treated adult rat DRG neurons in vitro were compared with NGF-depleted control neurons, SNS total mRNA levels and the levels of membrane-associated immunoreactive SNS showed a small increase (17 and 25%, respectively), while CGRP levels increased fourfold. SNS expression is thus little dependent on NGF even though SNS transcript levels dropped by more than 60% 7-14 days after axotomy. In the streptozotocin diabetic rat SNS levels fell 25%, while in several manipulations of the L5/6 tight nerve ligation rat neuropathic pain model, SNS levels fell 40-80% in rat strains that are either susceptible or relatively resistant to the development of allodynia. Increased expression of SNS mRNA is thus unlikely to underlie sensory neuron hyperexcitability associated with inflammation, while lowered SNS transcript levels are associated with peripheral nerve damage.

Original languageEnglish (US)
Pages (from-to)196-207
Number of pages12
JournalMolecular and Cellular Neurosciences
Volume10
Issue number3-4
DOIs
StatePublished - 1997
Externally publishedYes

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Sodium Channels
Neuralgia
Sensory Receptor Cells
Nerve Growth Factor
Neurons
Messenger RNA
Voltage-Gated Sodium Channels
Inflammation
Axotomy
Freund's Adjuvant
Diagnosis-Related Groups
Hyperalgesia
Tetrodotoxin
Spinal Ganglia
Streptozocin
Peripheral Nerves
Ligation
Membranes

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Okuse, K., Chaplan, S. R., McMahon, S. B., Luo, Z. D., Calcutt, N. A., Scott, B. P., ... Wood, J. N. (1997). Regulation of expression of the sensory neuron-specific sodium channel SNS in inflammatory and neuropathic pain. Molecular and Cellular Neurosciences, 10(3-4), 196-207. https://doi.org/10.1006/mcne.1997.0657

Regulation of expression of the sensory neuron-specific sodium channel SNS in inflammatory and neuropathic pain. / Okuse, Kenji; Chaplan, Sandra R.; McMahon, Stephen B.; Luo, Z. David; Calcutt, Nigel A.; Scott, Brian P.; Akopian, Armen N; Wood, John N.

In: Molecular and Cellular Neurosciences, Vol. 10, No. 3-4, 1997, p. 196-207.

Research output: Contribution to journalArticle

Okuse, Kenji ; Chaplan, Sandra R. ; McMahon, Stephen B. ; Luo, Z. David ; Calcutt, Nigel A. ; Scott, Brian P. ; Akopian, Armen N ; Wood, John N. / Regulation of expression of the sensory neuron-specific sodium channel SNS in inflammatory and neuropathic pain. In: Molecular and Cellular Neurosciences. 1997 ; Vol. 10, No. 3-4. pp. 196-207.
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