Regional variation in adipogenesis and IGF regulatory proteins in the fetal baboon

Yourka D. Tchoukalova, Peter W. Nathanielsz, Cheryl A. Conover, Steven R. Smith, Eric Ravussin

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Intrauterine growth rate is associated with body distribution in adulthood suggesting differential response of fetal fat depots to nutritional modifications. We hypothesize that there is regional differences in fetal adipogenesis, in part, due to depot-specific regulation of the availability of insulin growth factors. In near-term baboon fetuses (n = 3-5), the subcutaneous abdominal vs. omental preadipocytes had (1) more extensive lipid accumulation as assessed by BODIPY (lipid staining) to DAPI (nuclei) absorbance ratios (mean ± SEM; 0.51 ± 0.21, 0.35 ± 0.09, p < 0.05), (2) lower (p < 0.05) secretion of IGF-binding protein 4 (9.6 ± 1.2 vs. 17.4 ± 2.8 ng/ml) and its protease pregnancy associated plasma protein A (24.6 ± 1.9 vs. 39.1 ± 6.3 μIU/ml), (3) lower protein expression of IGF2 "clearance" receptor in cell lysate (0.28 ± 0.03 vs. 0.53 ± 0.02 OD U/mm2, p < 0.05); all variables were intermediate in femoral preadipocytes. The regional variation of the adipogenesis and the IGF regulatory pathway set the stage for differential responsiveness of fat depots to external signals.

Original languageEnglish (US)
Pages (from-to)679-683
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume380
Issue number3
DOIs
StatePublished - Mar 13 2009

Keywords

  • Adipogenesis
  • Adipose
  • Differentiation
  • Fat
  • Fetus
  • Insulin-like growth factor
  • Non-human primates
  • Preadipocyte
  • Pregnancy associated plasma protein

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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