Regional mapping of unique DNA sequences from human chromosome 3 derived from a flow-sorted chromosome library

Lakshmi M. Atchison, S. L. Naylor, J. J. Freed, R. L. Comis

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Eight single-copy DNA probes specific for human chromosome 3 were isolated by screening a human chromosome 3 derived genomic library. Southern blot analyses of DNAs isolated from a panel of somatic cell hybrids allowed us to regionally assign all probes to subregions on chromosome 3. Three clones were localized to the short arm of chromosome 3 (3p21→pier), two to the long arm (3q21→qter), and three to the 3q21→3p21 subregion. Six of these DNA sequences map to regions overlapping a segment of chromosome 3 (3p14→p23) frequently deleted in small cell lung cancer cells. Restriction fragment length polymorphism analyses indicate that at least three of the eight single-copy probes studied show Msp1 or Bg/II polymorphisms. This library is a useful source of chromosome 3 specific probes.

Original languageEnglish (US)
Pages (from-to)156-159
Number of pages4
JournalCytogenetics and Cell Genetics
Volume48
Issue number3
DOIs
StatePublished - 1988
Externally publishedYes

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Fingerprint

Dive into the research topics of 'Regional mapping of unique DNA sequences from human chromosome 3 derived from a flow-sorted chromosome library'. Together they form a unique fingerprint.

Cite this