Regional difference of ROS generation, lipid peroxidaton, and antioxidant enzyme activity in rat brain and their dietary modulation

Bong Sook Baek, Hyun Joo Kwon, Kyoung Hee Lee, Mi Ae Yoo, Kyu Won Kim, Yuji Ikeno, Byung Pal Yu, Hae Young Chung

Research output: Contribution to journalArticle

70 Scopus citations

Abstract

One of the potential causes of age-related neuronal damage can be reactive oxygen species (ROS), as the brain is particularly sensitive to oxidative damage. In the present study, we investigated the effects of aging and dietary restriction (DR) on ROS generation, lipid peroxidation, and antioxidant enzymes in cerebrum, hippocampus, and cerebellum of 6-, 12- 18-, and 24-month-old rats. ROS generation significantly increased with age in cerebrum of ad libitum (AL) rats. However, no significant age-difference was observed in hippocampus and cerebellum. DR significantly decreased ROS generation in cerebrum and cerebellum at 24-months. On the other hand, the increased lipid peroxidation of AL rats during aging was significantly reduced by DR in all regions. Our results further showed that catalase activity decreased with age in cerebellum of AL rats, which was reversed by DR, although SOD activity had little change by aging and DR in all regions. In a similar way, glutathione (GSH) peroxidase activity increased with age in cerebrum of AL rats, while DR suppressed it at 24-months. These data further support the evidence that the vulnerability to oxidative stress in the brain is region-specific.

Original languageEnglish (US)
Pages (from-to)361-366
Number of pages6
JournalArchives of Pharmacal Research
Volume22
Issue number4
DOIs
StatePublished - Aug 1999

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Keywords

  • Aging
  • Antioxidant enzymes
  • Brain
  • Dietary restriction
  • Lipid peroxidation
  • ROS generation

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery
  • Organic Chemistry

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