Regeneration of periodontal tissues in non-human primates with rhGDF-5 and beta-tricalcium phosphate

K. B. Emerton, S. J. Drapeau, H. Prasad, M. Rohrer, P. Roffe, K. Hopper, J. Schoolfield, Archie A Jones, David L Cochran

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The application of growth factors has been advocated in support of periodontal regeneration. Recombinant human growth and differentiation factor-5 (rhGDF-5), a member of the bone morphogenetic protein family, has been used to encourage periodontal tissue regeneration. This study evaluated the dose response of rhGDF-5 lyophilized onto beta-tricalcium phosphate (bTCP) granules for periodontal tissue regeneration in a baboon model. Periodontal defects were created bilaterally in 12 baboons by a split-mouth design. Plaque was allowed to accumulate around wire ligatures to create chronic disease. After 2 mos, the ligatures were removed, and a notch was placed at the base of the defect. Two teeth on each side of the mouth were randomly treated with bTCP only, 0.5, 1.0, or 2.0 mg rhGDF-5/g bTCP. Animals were sacrificed 5 mos post-treatment, with micro-CT and histomorphometric analysis performed. After 5 mos, analysis showed alveolar bone, cementum, and periodontal ligament formation in all treatment groups, with a dose-dependent increase in rhGDF-5-treated groups. Height of periodontal tissues also increased with the addition of rhGDF-5, and the amount of residual graft material decreased with rhGDF-5 treatment. Therefore, rhGDF-5 delivered on bTCP demonstrated effective regeneration of all 3 tissues critical for periodontal repair.

Original languageEnglish (US)
Pages (from-to)1416-1421
Number of pages6
JournalJournal of Dental Research
Volume90
Issue number12
DOIs
StatePublished - Dec 2011

Fingerprint

Primates
Regeneration
Papio
Ligation
Mouth
Dental Cementum
Periodontal Ligament
Bone Morphogenetic Proteins
beta-tricalcium phosphate
human GDF5 protein
Intercellular Signaling Peptides and Proteins
Tooth
Chronic Disease
Transplants
Bone and Bones

Keywords

  • biological factors
  • bone regeneration
  • GDF-5
  • medical devices
  • periodontal disease
  • periodontal regeneration

ASJC Scopus subject areas

  • Dentistry(all)

Cite this

Regeneration of periodontal tissues in non-human primates with rhGDF-5 and beta-tricalcium phosphate. / Emerton, K. B.; Drapeau, S. J.; Prasad, H.; Rohrer, M.; Roffe, P.; Hopper, K.; Schoolfield, J.; Jones, Archie A; Cochran, David L.

In: Journal of Dental Research, Vol. 90, No. 12, 12.2011, p. 1416-1421.

Research output: Contribution to journalArticle

Emerton, KB, Drapeau, SJ, Prasad, H, Rohrer, M, Roffe, P, Hopper, K, Schoolfield, J, Jones, AA & Cochran, DL 2011, 'Regeneration of periodontal tissues in non-human primates with rhGDF-5 and beta-tricalcium phosphate', Journal of Dental Research, vol. 90, no. 12, pp. 1416-1421. https://doi.org/10.1177/0022034511423665
Emerton, K. B. ; Drapeau, S. J. ; Prasad, H. ; Rohrer, M. ; Roffe, P. ; Hopper, K. ; Schoolfield, J. ; Jones, Archie A ; Cochran, David L. / Regeneration of periodontal tissues in non-human primates with rhGDF-5 and beta-tricalcium phosphate. In: Journal of Dental Research. 2011 ; Vol. 90, No. 12. pp. 1416-1421.
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