Reference transcriptome for deriving marmoset induced pluripotent stem cells

Guang Yang, Hyenjong Hong, April Torres, Kristen E. Malloy, Gourav Roy-Choudhury, Jeffrey Kim, Marcel M. Daadi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Limited access to primary tissue from various nonhuman primate (NHP) species represents a significant unmet need that hampers progress in understanding unique cellular diversity and gene regulation of specific tissues and organs in stem cell translational research. Most comparative biology studies have been limited to using postmortem tissue usually frozen specimens with limited utility for research. The generation of induced pluripotent stem cell (iPSC) lines from somatic cells, such as adult skin or blood cells, offers an alternative to invasive and ethically controversial interventions for acquiring tissue. Pluripotent iPSCs have virtually an unlimited capacity to proliferate and differentiate into all cell types of the body. We are generating high-quality validated NHP iPSC lines to offer to scientific community and facilitate their research programs. We use the non-integrative episomal vector system to generate iPSCs from NHP skin biopsies. In this chapter we describe the validation of NHP iPSC lines by confirming pluripotency and their propensity to differentiate into all three germ layers ectoderm, mesoderm, and endoderm according to established standards and measurable limits for a set of marker genes incorporated into a scorecard.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages175-186
Number of pages12
DOIs
StatePublished - 2019

Publication series

NameMethods in Molecular Biology
Volume1919
ISSN (Print)1064-3745

Keywords

  • iPSC characterization
  • Marmoset iPSCs
  • Nonhuman primate iPSCs
  • Pluripotency
  • Regenerative medicine
  • Scorecard

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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