Redundant and unique roles of two enhancer elements in the TCRγ locus in gene regulation and γδ T cell development

Na Xiong, Chulho Kang, David H. Raulet

Research output: Contribution to journalArticle

44 Scopus citations

Abstract

Many mammalian genes, including those encoding antigen receptors, contain more than one enhancer element. Deleting one element often does not prevent expression, but functional redundancy has never been directly demonstrated by gene targeting of multiple elements. We demonstrate that simultaneous deletion of two enhancer/LCR-like elements in the TCR Cγ1 cluster, HsA and 3′ECγ1, severely diminishes TCRγ transcription, selectively impairs development of γδ thymocyte subsets, but only modestly reduces TCRγ gene rearrangement, while deletion of each element separately has little effect. In contrast to these results in thymocytes, deletion of HsA alone reduces transcription of one Vγ gene specifically in peripheral γδ T cells. Thus, the two elements exhibit functional redundancy in thymocytes but also have unique functions in other settings.

Original languageEnglish (US)
Pages (from-to)453-463
Number of pages11
JournalImmunity
Volume16
Issue number3
DOIs
StatePublished - Jan 1 2002
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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