Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2

Guang Yang, Yan Dao Gong, Kai Gong, Wu Ling Jiang, Elaine Kwon, Pei Wang, Hui Zheng, Xiu Fang Zhang, Wen Biao Gan, Nan Ming Zhao

Research output: Contribution to journalArticle

75 Citations (Scopus)

Abstract

Although abnormal processing of amyloid precursor protein (APP) leads to early onset of Alzheimer's disease, the normal function of this protein is poorly understood. APP is widely expressed in axons, dendrites, and synapses in both central and peripheral nervous systems. Mice homozygous for APP or its homologue APP-like protein 2 (APLP2) null mutation (KO) are viable, but double mutants for APP and APLP2 deletions (DKO) are early postnatal lethal. To investigate the role of APP in synapse development, we compared the ultrastructure of submandibular ganglion synapses between DKO and littermate APLP2 KO mice at birth. Using serial electron microscopy, we found that the size of presynaptic boutons and the number of active zones per bouton were comparable in both strains of animals. However, the synaptic vesicle density, active zone size, and docked vesicle number per active zone were significantly reduced in DKO compared to those in APLP2 KO. These results indicate that the APP family of proteins plays an important role in regulating the formation and function of inter-neuronal synapses.

Original languageEnglish (US)
Pages (from-to)66-71
Number of pages6
JournalNeuroscience Letters
Volume384
Issue number1-2
DOIs
StatePublished - Aug 12 2005
Externally publishedYes

Fingerprint

Amyloid beta-Protein Precursor
Synaptic Vesicles
Synapses
Proteins
Parasympathetic Ganglia
Peripheral Nervous System
Dendrites
Axons
Electron Microscopy
Alzheimer Disease
Central Nervous System
Parturition
Mutation

Keywords

  • Alzheimer's disease
  • Amyloid precursor protein
  • Amyloid precursor-like protein
  • Electron microscopy
  • Submandibular ganglion

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2. / Yang, Guang; Gong, Yan Dao; Gong, Kai; Jiang, Wu Ling; Kwon, Elaine; Wang, Pei; Zheng, Hui; Zhang, Xiu Fang; Gan, Wen Biao; Zhao, Nan Ming.

In: Neuroscience Letters, Vol. 384, No. 1-2, 12.08.2005, p. 66-71.

Research output: Contribution to journalArticle

Yang, Guang ; Gong, Yan Dao ; Gong, Kai ; Jiang, Wu Ling ; Kwon, Elaine ; Wang, Pei ; Zheng, Hui ; Zhang, Xiu Fang ; Gan, Wen Biao ; Zhao, Nan Ming. / Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2. In: Neuroscience Letters. 2005 ; Vol. 384, No. 1-2. pp. 66-71.
@article{6f715ce7116a4f08a6ae2135bb90f130,
title = "Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2",
abstract = "Although abnormal processing of amyloid precursor protein (APP) leads to early onset of Alzheimer's disease, the normal function of this protein is poorly understood. APP is widely expressed in axons, dendrites, and synapses in both central and peripheral nervous systems. Mice homozygous for APP or its homologue APP-like protein 2 (APLP2) null mutation (KO) are viable, but double mutants for APP and APLP2 deletions (DKO) are early postnatal lethal. To investigate the role of APP in synapse development, we compared the ultrastructure of submandibular ganglion synapses between DKO and littermate APLP2 KO mice at birth. Using serial electron microscopy, we found that the size of presynaptic boutons and the number of active zones per bouton were comparable in both strains of animals. However, the synaptic vesicle density, active zone size, and docked vesicle number per active zone were significantly reduced in DKO compared to those in APLP2 KO. These results indicate that the APP family of proteins plays an important role in regulating the formation and function of inter-neuronal synapses.",
keywords = "Alzheimer's disease, Amyloid precursor protein, Amyloid precursor-like protein, Electron microscopy, Submandibular ganglion",
author = "Guang Yang and Gong, {Yan Dao} and Kai Gong and Jiang, {Wu Ling} and Elaine Kwon and Pei Wang and Hui Zheng and Zhang, {Xiu Fang} and Gan, {Wen Biao} and Zhao, {Nan Ming}",
year = "2005",
month = "8",
day = "12",
doi = "10.1016/j.neulet.2005.04.040",
language = "English (US)",
volume = "384",
pages = "66--71",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "1-2",

}

TY - JOUR

T1 - Reduced synaptic vesicle density and active zone size in mice lacking amyloid precursor protein (APP) and APP-like protein 2

AU - Yang, Guang

AU - Gong, Yan Dao

AU - Gong, Kai

AU - Jiang, Wu Ling

AU - Kwon, Elaine

AU - Wang, Pei

AU - Zheng, Hui

AU - Zhang, Xiu Fang

AU - Gan, Wen Biao

AU - Zhao, Nan Ming

PY - 2005/8/12

Y1 - 2005/8/12

N2 - Although abnormal processing of amyloid precursor protein (APP) leads to early onset of Alzheimer's disease, the normal function of this protein is poorly understood. APP is widely expressed in axons, dendrites, and synapses in both central and peripheral nervous systems. Mice homozygous for APP or its homologue APP-like protein 2 (APLP2) null mutation (KO) are viable, but double mutants for APP and APLP2 deletions (DKO) are early postnatal lethal. To investigate the role of APP in synapse development, we compared the ultrastructure of submandibular ganglion synapses between DKO and littermate APLP2 KO mice at birth. Using serial electron microscopy, we found that the size of presynaptic boutons and the number of active zones per bouton were comparable in both strains of animals. However, the synaptic vesicle density, active zone size, and docked vesicle number per active zone were significantly reduced in DKO compared to those in APLP2 KO. These results indicate that the APP family of proteins plays an important role in regulating the formation and function of inter-neuronal synapses.

AB - Although abnormal processing of amyloid precursor protein (APP) leads to early onset of Alzheimer's disease, the normal function of this protein is poorly understood. APP is widely expressed in axons, dendrites, and synapses in both central and peripheral nervous systems. Mice homozygous for APP or its homologue APP-like protein 2 (APLP2) null mutation (KO) are viable, but double mutants for APP and APLP2 deletions (DKO) are early postnatal lethal. To investigate the role of APP in synapse development, we compared the ultrastructure of submandibular ganglion synapses between DKO and littermate APLP2 KO mice at birth. Using serial electron microscopy, we found that the size of presynaptic boutons and the number of active zones per bouton were comparable in both strains of animals. However, the synaptic vesicle density, active zone size, and docked vesicle number per active zone were significantly reduced in DKO compared to those in APLP2 KO. These results indicate that the APP family of proteins plays an important role in regulating the formation and function of inter-neuronal synapses.

KW - Alzheimer's disease

KW - Amyloid precursor protein

KW - Amyloid precursor-like protein

KW - Electron microscopy

KW - Submandibular ganglion

UR - http://www.scopus.com/inward/record.url?scp=20444466355&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=20444466355&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2005.04.040

DO - 10.1016/j.neulet.2005.04.040

M3 - Article

C2 - 15919150

AN - SCOPUS:20444466355

VL - 384

SP - 66

EP - 71

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 1-2

ER -