Reduced live organism recovery and lack of hydrosalpinx in mice infected with plasmid-free Chlamydia muridarum

Lei Lei, Jianlin Chen, Shuping Hou, Yiling Ding, Zhangsheng Yang, Hao Zeng, Joel Baseman, Guangming Zhong

Research output: Contribution to journalArticlepeer-review

56 Scopus citations

Abstract

Plasmid-free Chlamydia trachomatis and Chlamydia muridarum fail to induce severe pathology. To evaluate whether the attenuated pathogenicity is due to insufficient infection or inability of the plasmidless chlamydial organisms to trigger pathological responses, we compared plasmid-competent and plasmid-free C. muridarum infections in 5 different strains of mice. All 5 strains developed hydrosalpinx following intravaginal inoculation with plasmid-competent, but not inoculation with plasmidfree, C. muridarum. The lack of hydrosalpinx induction by plasmid-free C. muridarum correlated with significantly reduced live organism recovery from the lower genital tract and shortened infection in the upper genital tract. The plasmid-free C. muridarum organisms failed to induce hydrosalpinx even when the organisms were directly inoculated into the oviduct via an intrabursal injection, which was accompanied by significantly reduced survival of the plasmidless organisms in the genital tracts. Furthermore, plasmid-competent C. muridarum organisms after UV inactivation were no longer able to induce hydrosalpinx even when directly delivered into the oviduct at a high dose. Together, these observations suggest that decreased survival of and shortened infection with plasmid-free C. muridarum may contribute significantly to its attenuated pathogenicity. We conclude that adequate live chlamydial infection in the oviduct may be necessary to induce hydrosalpinx.

Original languageEnglish (US)
Pages (from-to)983-992
Number of pages10
JournalInfection and Immunity
Volume82
Issue number3
DOIs
StatePublished - Mar 1 2014

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Reduced live organism recovery and lack of hydrosalpinx in mice infected with plasmid-free Chlamydia muridarum'. Together they form a unique fingerprint.

Cite this