Redox responses in yeast to acetate as the carbon source

Karyl I. Minard, L. McAlister-Henn

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

Following a shift to medium with acetate as the carbon source, a parental yeast strain exhibited a transient moderate 20% reduction in total cellular [NAD+ + NADH] but showed a ∼10-fold increase in the ratio of [NAD+]:[NADH] after 36 h. A mutant strain (idhΔ) lacking the tricarboxylic acid cycle enzyme isocitrate dehydrogenase had 50% higher cellular levels of [NAD+ + NADH] relative to the parental strain but exhibited similar changes in cofactor concentrations following a shift to acetate medium, despite an inability to grow on that carbon source; essentially all of the cofactor was in the oxidized form within 36 h. The salvage pathway for NAD(H) biosynthesis was found to be particularly important for viability during early transition of the parental strain to stationary phase in acetate medium. However, oxygen consumption was not affected, suggesting that the NAD(H) produced during this time may support other cellular functions. The idhΔ mutant exhibited increased flux through the salvage pathway in acetate medium but was dependent on the de novo pathway for viability. Long-term chronological lifespans of the parental and idhΔ strains were similar, but viability of the mutant strain was dependent on both pathways for NAD(H) biosynthesis.

Original languageEnglish (US)
Pages (from-to)136-143
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume483
Issue number1
DOIs
StatePublished - Mar 1 2009

Keywords

  • Chronological lifespan
  • Isocitrate dehydrogenase
  • NAD(H) levels
  • Redox status in stationary phase
  • Redox status in yeast cells
  • Salvage and de novo pathways for NAD(H) synthesis
  • Tricarboxylic acid cycle

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Redox responses in yeast to acetate as the carbon source'. Together they form a unique fingerprint.

Cite this